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I'm using the nextflow cut&run pipeline which I used last year for cut&run analysis. I'm now getting an error from the mark duplicates step citing 'handling an unexpected condition' and giving the exit status 140. I can't seem to find anything online regarding similar issues.
Command used and terminal output
Apr-30 12:38:04.698 [Task monitor] DEBUG nextflow.processor.TaskProcessor - Handling unexpected condition for task: name=NFCORE_CUTANDRUN:CUTANDRUN:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES (AKP_NT_Igg_H_PC_R2); work-dir=/exports/eddie/scratch/s1804715/cutandrun/work/5d/fb64d790e2eac4cb8df9b14d6d6f30 error [nextflow.exception.ProcessFailedException]: Process `NFCORE_CUTANDRUN:CUTANDRUN:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES (AKP_NT_Igg_H_PC_R2)` terminated with an error exit status (140)Apr-30 12:38:04.744 [Task monitor] ERROR nextflow.processor.TaskProcessor - Error executing process > 'NFCORE_CUTANDRUN:CUTANDRUN:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES (AKP_NT_Igg_H_PC_R2)'Caused by: Process `NFCORE_CUTANDRUN:CUTANDRUN:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES (AKP_NT_Igg_H_PC_R2)` terminated with an error exit status (140)Command executed: picard \ -Xmx64g \ MarkDuplicates \ --ASSUME_SORT_ORDER coordinate --REMOVE_DUPLICATES false --VALIDATION_STRINGENCY LENIENT --TMP_DIR tmp \ --INPUT AKP_NT_Igg_H_PC_R2.target.filtered.sorted.bam \ --OUTPUT AKP_NT_Igg_H_PC_R2.target.markdup.bam \ --REFERENCE_SEQUENCE genome.fa \ --METRICS_FILE AKP_NT_Igg_H_PC_R2.target.markdup.MarkDuplicates.metrics.txt cat <<-END_VERSIONS > versions.yml "NFCORE_CUTANDRUN:CUTANDRUN:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES": picard: $(echo $(picard MarkDuplicates --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) END_VERSIONSCommand exit status: 140Command output: (empty)Command error: ^[[34mINFO: ^[[0m Convert SIF file to sandbox... /usr/local/bin/picard: line 5: warning: setlocale: LC_ALL: cannot change locale (en_US.UTF-8): No such file or directory 11:38:01.799 INFO NativeLibraryLoader - Loading libgkl_compression.so from jar:file:/usr/local/share/picard-2.27.4-0/picard.jar!/com/intel/gkl/native/libgkl_compression.so [Tue Apr 30 11:38:01 GMT 2024] MarkDuplicates --INPUT AKP_NT_Igg_H_PC_R2.target.filtered.sorted.bam --OUTPUT AKP_NT_Igg_H_PC_R2.target.markdup.bam --METRICS_FILE AKP_NT_Igg_H_PC_R2.target.markdup.MarkDuplicates.metrics.txt --REMOVE_DUPLICATES false --ASSUME_SORT_ORDER coordi$ [Tue Apr 30 11:38:01 GMT 2024] Executing as [email protected] on Linux 3.10.0-1160.108.1.el7.x86_64 amd64; OpenJDK 64-Bit Server VM 11.0.15-internal+0-adhoc..src; Deflater: Intel; Inflater: Intel; Provider GCS is not available; Picard version: Version:2.27.4-SN$ INFO 2024-04-30 11:38:01 MarkDuplicates Start of doWork freeMemory: 108826936; totalMemory: 117440512; maxMemory: 68719476736 INFO 2024-04-30 11:38:01 MarkDuplicates Reading input file and constructing read end information. INFO 2024-04-30 11:38:01 MarkDuplicates Will retain up to 248983611 data points before spilling to disk.
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System information
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The text was updated successfully, but these errors were encountered:
Description of the bug
I'm using the nextflow cut&run pipeline which I used last year for cut&run analysis. I'm now getting an error from the mark duplicates step citing 'handling an unexpected condition' and giving the exit status 140. I can't seem to find anything online regarding similar issues.
Command used and terminal output
Relevant files
No response
System information
No response
The text was updated successfully, but these errors were encountered: