diff --git a/modules/nf-core/mirtop/counts/environment.yml b/modules/nf-core/mirtop/counts/environment.yml
index 82e42c1b4d5..0f0700ce301 100644
--- a/modules/nf-core/mirtop/counts/environment.yml
+++ b/modules/nf-core/mirtop/counts/environment.yml
@@ -6,7 +6,5 @@ channels:
   - bioconda
 dependencies:
   - "bioconda::mirtop=0.4.25"
-  - "bioconda::samtools=1.15.1"
   - "conda-forge::r-base=4.1.1"
   - "conda-forge::r-data.table=1.14.2"
-  - python=3.11
diff --git a/modules/nf-core/mirtop/export/environment.yml b/modules/nf-core/mirtop/export/environment.yml
index 2c4a751145d..5eee9fdadf4 100644
--- a/modules/nf-core/mirtop/export/environment.yml
+++ b/modules/nf-core/mirtop/export/environment.yml
@@ -9,4 +9,3 @@ dependencies:
   - "bioconda::samtools=1.15.1"
   - "conda-forge::r-base=4.1.1"
   - "conda-forge::r-data.table=1.14.2"
-  - python=3.11
diff --git a/modules/nf-core/mirtop/gff/environment.yml b/modules/nf-core/mirtop/gff/environment.yml
index 9f3a6f6dad2..c1104d3e39a 100644
--- a/modules/nf-core/mirtop/gff/environment.yml
+++ b/modules/nf-core/mirtop/gff/environment.yml
@@ -7,6 +7,3 @@ channels:
 dependencies:
   - "bioconda::mirtop=0.4.25"
   - "bioconda::samtools=1.15.1"
-  - "conda-forge::r-base=4.1.1"
-  - "conda-forge::r-data.table=1.14.2"
-  - python=3.11
diff --git a/modules/nf-core/mirtop/stats/environment.yml b/modules/nf-core/mirtop/stats/environment.yml
index 1f98726ccdc..13f3e637a84 100644
--- a/modules/nf-core/mirtop/stats/environment.yml
+++ b/modules/nf-core/mirtop/stats/environment.yml
@@ -9,4 +9,3 @@ dependencies:
   - "bioconda::samtools=1.15.1"
   - "conda-forge::r-base=4.1.1"
   - "conda-forge::r-data.table=1.14.2"
-  - python=3.11