diff --git a/CHANGELOG.md b/CHANGELOG.md index 00027a8cc..a2595690f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,7 +5,11 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## [dev] -- [PR #993] Added NGSCheckMate for checking that samples come from the same individual +### Enhancements and fixes + +- [PR #1126](https://github.com/nf-core/rnaseq/pull/1126) - Fixes error when transcript_fasta not provided and skip_gtf_filter set to true +- [#1125](https://github.com/nf-core/rnaseq/issues/1125) - Pipeline fails if transcript_fasta not provided and skip_gtf_filter = true +- [PR #993](https://github.com/nf-core/rnaseq/pull/993) Added NGSCheckMate for checking that samples come from the same individual ### Parameters @@ -28,7 +32,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Enhancements and fixes -- [[PR #1121](https://github.com/nf-core/rnaseq/pull/1121) - Changes for 3.13.1 patch release incl. igenomes star fix +- [PR #1121](https://github.com/nf-core/rnaseq/pull/1121) - Changes for 3.13.1 patch release incl. igenomes star fix ## [[3.13.0](https://github.com/nf-core/rnaseq/releases/tag/3.13.0)] - 2023-11-17 diff --git a/subworkflows/local/prepare_genome/main.nf b/subworkflows/local/prepare_genome/main.nf index 0be947954..0840c7734 100644 --- a/subworkflows/local/prepare_genome/main.nf +++ b/subworkflows/local/prepare_genome/main.nf @@ -47,7 +47,7 @@ workflow PREPARE_GENOME { rsem_index // directory: /path/to/rsem/index/ salmon_index // directory: /path/to/salmon/index/ kallisto_index // directory: /path/to/kallisto/index/ - hisat2_index // directory: /path/to/hisat2/index/ + hisat2_index // directory: /path/to/hisat2/index/ bbsplit_index // directory: /path/to/rsem/index/ gencode // boolean: whether the genome is from GENCODE is_aws_igenome // boolean: whether the genome files are from AWS iGenomes @@ -139,14 +139,13 @@ workflow PREPARE_GENOME { } else { ch_transcript_fasta = Channel.value(file(transcript_fasta)) } - if (gencode) { + if (gencode) { PREPROCESS_TRANSCRIPTS_FASTA_GENCODE ( ch_transcript_fasta ) ch_transcript_fasta = PREPROCESS_TRANSCRIPTS_FASTA_GENCODE.out.fasta ch_versions = ch_versions.mix(PREPROCESS_TRANSCRIPTS_FASTA_GENCODE.out.versions) } } else { ch_transcript_fasta = MAKE_TRANSCRIPTS_FASTA ( ch_fasta, ch_gtf ).transcript_fasta - ch_versions = ch_versions.mix(GTF_FILTER.out.versions) ch_versions = ch_versions.mix(MAKE_TRANSCRIPTS_FASTA.out.versions) } @@ -268,7 +267,7 @@ workflow PREPARE_GENOME { ch_versions = ch_versions.mix(SALMON_INDEX.out.versions) } } - + // // Uncompress Kallisto index or generate from scratch if required //