[NTR] CD66acde-positive enterocyte described by Stellar pipeline snRNAseq + CODEX/Phenocycler John Hickey

[emquardokus]

Please check that the term does not already exist by using the ontology search tool OLS:
https://www.ebi.ac.uk/ols4/ontologies/cl

Preferred term label

• CD66acde-positive enterocyte

Synonyms (add reference(s), please)

Definition (free text, with reference(s), please. PubMed ID format is PMID:XXXXXX)

(The antibody used was for CD66acde see: https://www.bdbiosciences.com/en-us/products/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/purified-mouse-anti-human-cd66.551354)
Description:
Definition: An enterocyte of the human colon (large intestine) expressing carcinoembryonic antigen-related cell adhesion molecules 1, 3, 5, and 6 (CEACAM1, CEACAM3, CEACAM5, CEACAM6) , also known as CD66acde.
Localization: Predominantly found in the colon at the top of the mucosa, representing mature enterocyte populations.
Characteristics: These cells are characterized by the expression of CD66acde, indicating a mature state and specific functional properties within the colonic epithelium.
Significance: CD66acde is involved in cell adhesion and intracellular signaling. Its expression on enterocytes signifies maturation and possibly roles in barrier function and cell-cell communication (CD66 is also present on neutrophils but CD66 + Pan cytokeratin will indicate epithelial while CD66 + CD45 will indicate neutrophils).
Note on CEACAM Subunits:
In our study, CD66acde (CEACAM1, CEACAM3, CEACAM5, CEACAM6) were the specific subunits used to identify these enterocytes.
Definition for Cell Ontology:
CD66acde-positive enterocyte: An enterocyte of the human colon expressing carcinoembryonic antigen-related cell adhesion molecule 1, 3, 5, and 6 (CEACAM1, CEACAM3, CEACAM5, CEACAM6), also known as CD66acde. These cells are mature enterocytes with a distinct expression profile compared to other enterocytes and are prevalent in the colonic epithelium.

Hickey, John W., Winston R. Becker, Stephanie A. Nevins, Aaron Horning, Almudena Espin Perez, Chenchen Zhu, Bokai Zhu, et al. 2023. “Organization of the Human Intestine at Single-Cell Resolution.” Nature 619 (7970): 572–84. https://doi.org/10.1038/s41586-023-05915-x.

Parent cell type term (check the hierarchy here https://www.ebi.ac.uk/ols4/ontologies/cl)
enterocyte of epithelium of large intestine CL:0002071

Anatomical structure where the cell type is found (check Uberon for anatomical structures: https://www.ebi.ac.uk/ols4/ontologies/uberon)
colon UBERON:0001155

Your ORCID
Ellen M. Quardokus 0000-0001-7655-4833
John W. Hickey 0000-0001-9961-7673

Additional notes or concerns
Additional Clarifications
CD66a-positive enterocytes: Predominantly in the colon (large intestine).
Markers Associated with Immune Cells:
While CD66a is traditionally associated with immune cells (e.g., neutrophils), its expression on enterocytes marks unique epithelial subsets with specific functions or maturation states.
Disease Association:
Hickey et al 2023. Nature 619 (7970): 572–84. study focused on healthy intestinal tissues. The expression of this marker in enterocytes within healthy samples suggests that they are part of normal physiological processes rather than disease states.
Summary
We identified this enterocyte type to capture the heterogeneity within the intestinal epithelium:
CD66a-positive enterocytes denote mature enterocytes prevalent in the colon.

[aleixpuigb]

Hi @emquardokus,

I am a bit uncertain wether to add this cell type. The antibody used recognizes 4 different epitopes (CD66a, c, d and e). That could mean that the antibody is identifying different cell types (e.g. some only express CD66a and other CD66d), is that correct? Moreover, calling it CD66-positive is misleading, because we are not certain that it is expressing all the CD66 family members. I think we need some more information for these cells.