The original wetlab protocol for OCTOPUS 3.0 can be found on our Bench Protocol page. If you have an Opentrons OT-2, check out how it can be set up to assist in library prep.
We introduced improvements to RCA (rolling circle amplification) in OCTOPUS that specifically enrich for plasmid reads over other contaminants. We highly recommend this "focused" RCA approach, which you can read about here.
Browse these resources for more information on our wetlab protocol:
You can refer to these resources for information on our computational protocol: