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Multiple PlateAcquisitions: Images in wrong Wells #96

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will-moore opened this issue Sep 6, 2024 · 1 comment
Open

Multiple PlateAcquisitions: Images in wrong Wells #96

will-moore opened this issue Sep 6, 2024 · 1 comment

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@will-moore
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See IDR/idr-metadata#683 (comment)

In the first Acquistion (see screenshot) the first 6 adjacent Wells show Images from the 6 Fields (acquisitions) from A1, then A2, the (2nd row) A3, A4 etc.

Screenshot 2024-08-30 at 12 04 03

@sbesson
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sbesson commented Sep 9, 2024

Feels like there is a mismatch between the Image.series stored in the database (and populated during the initial import using the IDR FLexReader) and the series set internally by this reader.

Looking at the metadata for first images in the first plate acquisition in 1229801, 1229801, 1229921, the series are 0, 1, 2...

An additional check would be whether importing the Zarr plate from scratch has a similar ordering issue or if it only manifests when replacing the Fileset. If a standard import works as expected, I think there are two available options:

  • updating ZarrReader to enforce the series ordering for Plate with multiple acquisitions is consistent with the decisions made for idr0001
  • updating image.series in the database to match the series order enforced in ZarrReader.

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