Beads.md

title	layout	description
Beads	page	Types of fluorescent beads

This page is about fluorescent beads, used as fiduciary markers for multi-view registration in light sheet microscopy.

Estapor Fluorescent Microspheres

• website
• stock concentration for SPIM: 14 µl 0.5 µm beads in 14 ml medium (sample dependent, e.g. PBS/T for fruit fly, E3 for zebrafish)

Tips & tricks

• extended vortexing and/or treatment in ultrasonic bath is crucial for homogeneous bead distribution and successful bead detection
• evaluate the bead concentration in agarose w/o specimen

F-XC050

• diameter 0.5 µm
• "green" emission (see spectra)
• EX 488 nm
• applications:
  • fiduciary marker in "green" detection channel for bright "green" fluorescence
  • evaluation/adjustment of red-green overlay

F-Y050

• diameter 0.5 µm
• "yellow" emission (see spectra)
• EX 488/561 nm
• applications:
  • fiduciary marker in "green" detection channel for weak "green" fluorescence
  • fiduciary marker in "red" detection channel for "green" fluorescence

Spectral properties

{% include image src="Estapor-beads.png" width="100%" caption="Emission spectra" %}