RNA isoform/alternative splicing analysis

[grimwoo]

Hi, Kallisto/BUSTools was recommended by Seurat to perform RNA isoform/alternative splicing analysis. However, I didn't find the related workflow and found nothing in GitHub with the key word "isoform". This may due to my poor understanding of this package. Coud anyone help me ?

[sbooeshaghi]

What is the datatype, 3' or full length (ie Smart-seq?)

[grimwoo]

@sbooeshaghi Thank you for the early reply. The datatype is 10x single cell RNA seq ( 3').

[LuoJulia]

Hi, was just wondering if there's been any update to this question? I'm interested in alternative splicing analysis as well.

[tstannius]

Dear Devs

I am also interested in alternative splicing analysis (10X with hashtag oligo data) :-)

[gadepallivs]

@sbooeshaghi Hello, is there any update on the Differential expression with equivalence classes in TCC matrix (coming soon) using the kb-python ?