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MFEprimer 3.0: PCR Primer Quality Control

Introduction

The name "MFEprimer" comes from the title of the first paper (2009, Bioinformatics): Multiple Factor Evaluation of the specificity of PCR primers. And both the version 1.0 and version 2.0 (2012, NAR) are only focused on primer specificty, leaving dimers and hairpins for users. However, dimers and hairpins are very important factors affecting success of PCR. So, for version 3.0, we expand the functions of MFEprimer, and make it as a full-functional primer quality control (PrimerQC, maybe the new name) program.

Features of v3.0

1. genomewide specificity check;
2. new and fast dimer check algorithm;
3. new and fast hairpin check algorithm;
4. primers compatible check for multiplex PCR;
5. thermodynamics based hybridization analysis;
6. core algorithm was rewritten with golang, which is fast and parallel.

Support

1. iGeneTech Bioscience Co., Ltd.
2. Xiaolei Wang & Dr. Dongsheng Zhao