README.md

Overview

This repository hosts sample preparation protocols for repertoire sequencing

Structure

Each *.md or *.pdf file represents single protocol.

Protocols

• Extraction of mononuclear cells (MNCs) from periferal blood;
• Human TCR alpha and beta RNA-based RACE protocol with unique molecular barcoding;
• DNA isolation from low cell numbers;
• AMPure XP beads DNA purification;
• RNeasy MinElute Cleanup Handbook;
• Total RNA extraction using Trizol.