QC after nextclip ?

[ptranvan]

Hi,

Should we perform a QC step, with trimmomatic for instance, in order to filter out adaptor and trim low quality reads after a nextclip process ?

[richardmleggett]

Hi there,

Yes, I would say this is worth doing. However, I wouldn't set the quality threshold too high - use, say, Q20.

Thanks,
Richard

On 1 Jul 2016, at 14:32, ptranvan <[email protected]mailto:[email protected]> wrote:

Hi,

Should we perform a QC step, with trimmomatic for instance, in order to filter out adaptor and trim low quality reads after a nextclip process ?

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