diff --git a/Pipfile.lock b/Pipfile.lock
index cbdeac6..b530170 100644
--- a/Pipfile.lock
+++ b/Pipfile.lock
@@ -186,26 +186,26 @@
         },
         "cutadapt": {
             "hashes": [
-                "sha256:0d2f7a7ce0d6325dd6f375150b1d42fa86e96d8d5fb3147e139891be04ffcea6",
-                "sha256:2778906e34fb2bf3ec686f151a36985da28146d86ed809bd4953f009c08ec941",
-                "sha256:2c730034ad1ccb9298cda09de03e3ed5f1b2ef9f9d2ea43765b9e51c53f92476",
-                "sha256:386294bdd6f7969b091fc09338b576e017ae4d953cc14a5f56a1b075feb9f707",
-                "sha256:38d4c26fc9970dcd8f47276aa4674415dc0f72597526898c3d5b8dc86f095ceb",
-                "sha256:5cbb478a4fb574d82f43b4d0bd67fb6dc08d89ed6feaad4edb0fe838ba1b8977",
-                "sha256:87a6eb655d6caf479dca7ba770ebd05d6612cdd76e9cebf6217164a86b075b79",
-                "sha256:924116f34569248035b16f58e73458ed4c0004e44823b80b07f4ab419272f591",
-                "sha256:957f00b464adf3f8012e904116918104269de1a92c56c55f14127a88bbc6aa1f",
-                "sha256:9aa22e4b4939ad6895f467457a91402fb58d1411e7db5ff27ccc80c5f683017a",
-                "sha256:ae44cebfceeacb5433f3f2df8101f08136a75323aa941af8e83bf59a84003f10",
-                "sha256:bfad09513502c0faa22ab8964776d8e2fd759813f24d9c51a48432894f7880be",
-                "sha256:ca52772ddba357cb4d41c57f9aa9b8ee3e2458f0075eae03ac9741a44e4589ef",
-                "sha256:cc0b581c92aaf1af9a3b5b213f1bd74d47e212db81f13fcf3df66208b01dce19",
-                "sha256:d3ca2f22deea2fbb55d27d23fff68c1d797ae3d5b30a7fc99a3823203d1e26f7",
-                "sha256:df0b7826691eaead60b40ecd7fdd34fcd2b97f7a19e81f87938341eeb96dec7d"
+                "sha256:143511433bda78275b2e5551b87eeff6ad5db23b9e8e1141da3059ac834bbb97",
+                "sha256:18f7aa6623bab2aba09567e93db0004b73346da34c589178486efe1b9121c47d",
+                "sha256:2ea523ec40e2e9f8091d3c0bc1b69c99c073d0dffe6bc843cceee4780414a009",
+                "sha256:30467bb9bd4d9855ddb31d42bc2732ca2077b88e0f46fd7da272f83750ebd90d",
+                "sha256:396a8193c490f52c58975d421ca4cb705cd862d2f7140003471abad2c0e86e72",
+                "sha256:41ccb116aefdc1b9e56a0c11dccee6d37b61fce11d1e9502d2d50089cab23d10",
+                "sha256:5db6878bc8fda9472cf881798173b868bbdbf7b955001ee727eac5eed53742b6",
+                "sha256:69e803b7e1ab0226246e60298d778a0e54596a5758d6b0a78d11736254215680",
+                "sha256:6c750e76bf6cb0c28a5658201a150d4fc142dbf121805e8e9131fcd03f8ffd65",
+                "sha256:7114ea84d8f97257162eff6cda48c13ba748aa822c8d183cd6302b489cb8a2bd",
+                "sha256:8ad7b5fea1fdf1d3bc184f674d18c3cfc2ab9ab370bbecf96b84237747b9930e",
+                "sha256:aa547e7236c660e5779ddf0abb58840b1dba81012e1ba5f8faf877bcb99f5ecd",
+                "sha256:ab334b66b88c994cb789f1956d7d454f08d6f9c4924bc0748897ca71d73c7b23",
+                "sha256:ac852f6b5f2d1147d0d34bef2eaa5879776f81c69a35dd328a701aae39ec6034",
+                "sha256:c25f0f093d491f039260a171ddd04f520a14691882119d244c79ba7c2c0ea7ce",
+                "sha256:e2260226aff84f9bbb9cdf97085dfe61fac466914cdf382354452f0df01004b5"
             ],
             "index": "pypi",
-            "markers": "python_version >= '3.7'",
-            "version": "==4.6"
+            "markers": "python_version >= '3.8'",
+            "version": "==4.8"
         },
         "dnaio": {
             "hashes": [
@@ -254,52 +254,52 @@
         },
         "isal": {
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             "markers": "platform_machine == 'x86_64' or platform_machine == 'AMD64' or platform_machine == 'aarch64'",
-            "version": "==1.6.0"
+            "version": "==1.6.1"
         },
         "natsort": {
             "hashes": [
@@ -852,60 +852,60 @@
         },
         "xopen": {
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