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lines changed Original file line number Diff line number Diff line change 99TensorFlow 2.15 itself requires python 3.11 or earlier.
1010
1111We use cellpose 3.x because cellpose 4.x is heavily biased towards using their `cpsam`,
12- "segment anything" model. This is a very cool model, but it is also huge and performance
13- with a CPU is not great. It is also more powerful than needed for this example.
12+ "segment anything" model. This is a very cool model, but it is also huge and may require GPU use.
13+ `cpsam` is also more powerful than needed for this example.
1414
1515Using cellpose 3.x allows us to stick with the light and focused `ctyo` model for segmentation.
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5151to remove StarDist and upgrade to Cellpose 4.x, which would be used to segment both cytoplasm and
5252nuclear channels.
5353
54+ NOTE: Because of the size of the cpsam model used in Cellpose 4.x, they advise against CPU-based segmentation.
55+ Using `gpu=True` with Cellpose models from the Fiji script editor is untested.
56+
5457==========================
5558Known Issues
5659==========================
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