ngs.plot.r

[Liyi56]

after install some packages, the information of error change to this,
Using database:
/share/workplace/home/stu_huiyanan/soft_python/ngsplot/database/IRGSP-1/IRGSP-1.ensembl.genebody.protein_coding.RData
Done
Loading R librariesWarning message:
multiple methods tables found for ‘rep.int’
.....Done
Analyze bam files and calculate coverage.Done
Plotting figures...Error: object 'g.order' not found
Execution halted

my command is # ngs.plot.r -G IRGSP-1 -R tss -C H2AZ-F1_R1.bam -O h2az.tss -T H2AZ -L 3000 -FL 300
I have a problem in run the ngs.plot.r. please save me！

[sorrowripple]

I suffered this question, too, but my reason is that I just provide one gene to plot.
ngs.plot.r can't cluster using one gene, when I provide more genes, it run without error.
and I think this error just influence the heatmap because my line plot is fine.
Hope my experience can help you.

[Liyi56]

very thanks for your valuable experience! Later, I choose another tools 'deeptools'.