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Input and output files #13

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pankaj209h opened this issue Feb 24, 2024 · 1 comment
Open

Input and output files #13

pankaj209h opened this issue Feb 24, 2024 · 1 comment

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@pankaj209h
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I want to predict the phage-host using the SPacePHARER a tool developed by your team. I have metatranscriptome data and want to screen the bacteriophages. My question is what will be the .fas file (spacepharer easy-predict examples/*.fas targetSetDB predictions.tsv tmpFolder). Also which databases are accurately helpful for this identification. Can I predict phage and host using this tool?

Look forward to your help and suggestions. I have raw .gz files from the metatranscriptomics as input.

@pankaj209h
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pankaj209h commented Feb 24, 2024

How to make Spacers file? Can I use the same .fas file (JNGQ01000029.fas and CP003088.fas) given in tutorial or I need to make new one. Please help me how to mke these files.
image

My predictmatch is showing failed
;
Query database size: 305 type: Aminoacid
Estimated memory consumption: 19G
Target database size: 25366234 type: Aminoacid
Index table k-mer threshold: 112 at k-mer size 6
Index table: counting k-mers
[=================================================================] 100.00% 25.37M 10m 22s 736ms
Index table: Masked residues: 17104442
Index table: fill
tmpFolder/8143349053704083950/predict/16566698282892545376/search/8965319943649311865/blastp.sh: line 99: 36 198 Bus error $RUNNER "$MMSEQS" prefilter "$INPUT" "$TARGET" "$TMP_PATH/pref_$STEP" $PREFILTER _PAR -s "$SENS"
Error: Prefilter died
Error: search failed
Error: predictmatch failed

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