diff --git a/src/strands/types/media.py b/src/strands/types/media.py
index 29b89e5c..b778337a 100644
--- a/src/strands/types/media.py
+++ b/src/strands/types/media.py
@@ -13,14 +13,33 @@
 """Supported document formats."""
 
 
-class DocumentSource(TypedDict):
+class S3Location(TypedDict, total=False):
+    """Contains the S3 location information for a document.
+
+    Attributes:
+        bucket: The S3 bucket name.
+        key: The S3 object key.
+
+    Note:
+        Both bucket and key are required for a valid S3 location,
+        but they are marked as optional in the type definition to allow
+        for runtime validation in the code.
+    """
+
+    bucket: str
+    key: str
+
+
+class DocumentSource(TypedDict, total=False):
     """Contains the content of a document.
 
     Attributes:
         bytes: The binary content of the document.
+        s3Location: The S3 location of the document (for Bedrock Nova models).
     """
 
     bytes: bytes
+    s3Location: S3Location
 
 
 class DocumentContent(TypedDict):
@@ -29,7 +48,7 @@ class DocumentContent(TypedDict):
     Attributes:
         format: The format of the document (e.g., "pdf", "txt").
         name: The name of the document.
-        source: The source containing the document's binary content.
+        source: The source containing the document's binary content or S3 location.
     """
 
     format: Literal["pdf", "csv", "doc", "docx", "xls", "xlsx", "html", "txt", "md"]
diff --git a/tests/strands/models/test_bedrock.py b/tests/strands/models/test_bedrock.py
index 1d045f3b..04914f8f 100644
--- a/tests/strands/models/test_bedrock.py
+++ b/tests/strands/models/test_bedrock.py
@@ -364,6 +364,51 @@ def test_format_request_cache(model, messages, model_id, tool_spec, cache_type):
     assert tru_request == exp_request
 
 
+def test_message_with_both_document_sources(bedrock_client, model):
+    """Test that messages with both S3 and bytes document sources are properly formatted."""
+    # Setup mock response
+    bedrock_client.converse_stream.return_value = {
+        "stream": [
+            {"messageStart": {"role": "assistant"}},
+            {"contentBlockDelta": {"delta": {"text": "I've analyzed both documents."}}},
+            {"contentBlockStop": {}},
+            {"messageStop": {"stopReason": "end_turn"}},
+        ]
+    }
+
+    # Create document with both source types (this is allowed by the type but might not be valid for the API)
+    document = {
+        "format": "pdf",
+        "name": "test.pdf",
+        "source": {
+            "bytes": b"test document content",
+            "s3Location": {"bucket": "test-bucket", "key": "documents/test.pdf"},
+        },
+    }
+
+    # Create message with the mixed document
+    messages = [{"role": "user", "content": [{"text": "Please analyze this document"}, document]}]
+
+    # Call converse and collect events
+    list(model.converse(messages))
+
+    # Verify the request was made with both document sources
+    args, kwargs = bedrock_client.converse_stream.call_args
+    request = kwargs
+
+    # Extract the document content from the request
+    request_messages = request["messages"]
+    user_message = request_messages[0]
+    document_content = user_message["content"][1]
+
+    # Verify both sources were included in the request
+    assert "bytes" in document_content["source"]
+    assert "s3Location" in document_content["source"]
+    assert document_content["source"]["bytes"] == b"test document content"
+    assert document_content["source"]["s3Location"]["bucket"] == "test-bucket"
+    assert document_content["source"]["s3Location"]["key"] == "documents/test.pdf"
+
+
 def test_format_chunk(model):
     tru_chunk = model.format_chunk("event")
     exp_chunk = "event"