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READLEN varaible used as MAX and MIN value #2

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bridlin opened this issue Nov 4, 2021 · 0 comments
Open

READLEN varaible used as MAX and MIN value #2

bridlin opened this issue Nov 4, 2021 · 0 comments

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@bridlin
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bridlin commented Nov 4, 2021

Hello!
The READLEN variable defined in the config file is used by cutadapt --minimum-length option to filter shorter reads out and by DADA2 as Trunclen option to cut the reads at this length.
This is a problem when there are amplicons that are shorter than the sequencing length as this will introduce variation in the expected read length.

Thanks Bridlin

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