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Hello!
The READLEN variable defined in the config file is used by cutadapt --minimum-length option to filter shorter reads out and by DADA2 as Trunclen option to cut the reads at this length.
This is a problem when there are amplicons that are shorter than the sequencing length as this will introduce variation in the expected read length.
Thanks Bridlin
The text was updated successfully, but these errors were encountered:
Hello!
The READLEN variable defined in the config file is used by cutadapt --minimum-length option to filter shorter reads out and by DADA2 as Trunclen option to cut the reads at this length.
This is a problem when there are amplicons that are shorter than the sequencing length as this will introduce variation in the expected read length.
Thanks Bridlin
The text was updated successfully, but these errors were encountered: