MS-DIAL 5.5 Crashes During Alignment

[JohnFu4]

Hello,

I am experiencing an issue where MS-DIAL 5.5 (and other versions 5.X) consistently crashes when I try to perform data alignment. These data work fine in MS-DIAL 4.9. Specifically, I can view features in individual samples; however, when I attempt to align all samples and view them together, the software crashes immediately.

I am using the following settings in MS-DIAL 5.5: proteomics mode, negative ion mode, with data measured in full scan mode on an LC-qTOF. The measured data are imported in mzML format.

In the Windows Event Viewer, I found the following report:

- 1026 2 0 0x80000000000000 1404124 Application CZBLKSYNTH660.cz.corporate.synthon-group.com - Application: MSDIAL.exe Framework Version: v4.0.30319 Description: The process was terminated due to an unhandled exception. Exception Info: System.ArgumentNullException at CompMs.MsdialCore.Parser.MsdialProteomicsSerializer.LoadProteinResultContainer(System.String) at CompMs.App.Msdial.Model.Lcms.LcmsAlignmentModel..ctor(CompMs.App.Msdial.Model.DataObj.AlignmentFileBeanModel, CompMs.MsdialCore.Algorithm.Annotation.IMatchResultEvaluator`1, CompMs.MsdialCore.DataObj.DataBaseStorage, CompMs.App.Msdial.Model.Search.PeakSpotFiltering`1, CompMs.App.Msdial.Model.Search.PeakFilterModel, CompMs.MsdialCore.DataObj.DataBaseMapper, CompMs.MsdialLcmsApi.Parameter.MsdialLcmsParameter, CompMs.App.Msdial.Model.DataObj.FilePropertiesModel, System.Collections.Generic.List`1, CompMs.App.Msdial.Model.DataObj.AnalysisFileBeanModelCollection, Reactive.Bindings.Notifiers.IMessageBroker) at CompMs.App.Msdial.Model.Lcms.LcmsMethodModel.LoadAlignmentFileCore(CompMs.App.Msdial.Model.DataObj.AlignmentFileBeanModel) at CompMs.App.Msdial.Model.Core.MethodModelBase.LoadAlignmentFileAsync(CompMs.App.Msdial.Model.DataObj.AlignmentFileBeanModel, System.Threading.CancellationToken) at CompMs.App.Msdial.ViewModel.Lcms.LcmsMethodViewModel.LoadAlignmentFileCoreAsync(CompMs.App.Msdial.ViewModel.DataObj.AlignmentFileBeanViewModel, System.Threading.CancellationToken) at CompMs.App.Msdial.ViewModel.Core.MethodViewModel.LoadAlignmentFileAsync(System.Threading.CancellationToken) at CompMs.App.Msdial.ViewModel.Core.MethodViewModel+<>c__DisplayClass0_0.<.ctor>b__11() at Reactive.Bindings.AsyncReactiveCommand+<>c__DisplayClass6_0+<b__0>d.MoveNext() at System.Runtime.ExceptionServices.ExceptionDispatchInfo.Throw() at System.Runtime.CompilerServices.TaskAwaiter.HandleNonSuccessAndDebuggerNotification(System.Threading.Tasks.Task) at Reactive.Bindings.AsyncReactiveCommand`1+d__16[[System.__Canon, mscorlib, Version=4.0.0.0, Culture=neutral, PublicKeyToken=b77a5c561934e089]].MoveNext() at System.Runtime.ExceptionServices.ExceptionDispatchInfo.Throw() at System.Runtime.CompilerServices.TaskAwaiter.HandleNonSuccessAndDebuggerNotification(System.Threading.Tasks.Task) at Reactive.Bindings.AsyncReactiveCommand`1+d__15[[System.__Canon, mscorlib, Version=4.0.0.0, Culture=neutral, PublicKeyToken=b77a5c561934e089]].MoveNext() at System.Runtime.ExceptionServices.ExceptionDispatchInfo.Throw() at System.Windows.Threading.ExceptionWrapper.InternalRealCall(System.Delegate, System.Object, Int32) at System.Windows.Threading.ExceptionWrapper.TryCatchWhen(System.Object, System.Delegate, System.Object, Int32, System.Delegate) at System.Windows.Threading.DispatcherOperation.InvokeImpl() at MS.Internal.CulturePreservingExecutionContext.CallbackWrapper(System.Object) at System.Threading.ExecutionContext.RunInternal(System.Threading.ExecutionContext, System.Threading.ContextCallback, System.Object, Boolean) at System.Threading.ExecutionContext.Run(System.Threading.ExecutionContext, System.Threading.ContextCallback, System.Object, Boolean) at System.Threading.ExecutionContext.Run(System.Threading.ExecutionContext, System.Threading.ContextCallback, System.Object) at MS.Internal.CulturePreservingExecutionContext.Run(MS.Internal.CulturePreservingExecutionContext, System.Threading.ContextCallback, System.Object) at System.Windows.Threading.DispatcherOperation.Invoke() at System.Windows.Threading.Dispatcher.ProcessQueue() at System.Windows.Threading.Dispatcher.WndProcHook(IntPtr, Int32, IntPtr, IntPtr, Boolean ByRef) at MS.Win32.HwndWrapper.WndProc(IntPtr, Int32, IntPtr, IntPtr, Boolean ByRef) at MS.Win32.HwndSubclass.DispatcherCallbackOperation(System.Object) at System.Windows.Threading.ExceptionWrapper.InternalRealCall(System.Delegate, System.Object, Int32) at System.Windows.Threading.ExceptionWrapper.TryCatchWhen(System.Object, System.Delegate, System.Object, Int32, System.Delegate) at System.Windows.Threading.Dispatcher.LegacyInvokeImpl(System.Windows.Threading.DispatcherPriority, System.TimeSpan, System.Delegate, System.Object, Int32) at MS.Win32.HwndSubclass.SubclassWndProc(IntPtr, Int32, IntPtr, IntPtr) at MS.Win32.UnsafeNativeMethods.DispatchMessage(System.Windows.Interop.MSG ByRef) at System.Windows.Threading.Dispatcher.PushFrameImpl(System.Windows.Threading.DispatcherFrame) at System.Windows.Application.RunDispatcher(System.Object) at System.Windows.Application.RunInternal(System.Windows.Window) at CompMs.App.Msdial.App.Main()

Thank you very much for your assistance.