diff --git a/CHANGELOG.md b/CHANGELOG.md
index efc5892..956c0b5 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -19,6 +19,7 @@
 - Add alias `--no-capture` for the option `-c` (gh-391).
 - Fix reporting of an assertion failure in `Server:exec()` in case verbose
   error serialization is enabled in Tarantool (gh-376).
+- Added `assert_items_exclude`.
 
 ## 1.0.1
 
diff --git a/README.rst b/README.rst
index c99a245..183b2db 100644
--- a/README.rst
+++ b/README.rst
@@ -239,6 +239,9 @@ List of luatest functions
 +--------------------------------------------------------------------+-----------------------------------------------+
 | ``assert_eval_to_true (value[, message])``                         | Alias for assert.                             |
 +--------------------------------------------------------------------+-----------------------------------------------+
+| ``assert_items_exclude (actual, expected[, message])``             | Checks that one table does not include any    |
+|                                                                    | items of another, irrespective of their keys. |
++--------------------------------------------------------------------+-----------------------------------------------+
 | ``assert_items_include (actual, expected[, message])``             | Checks that one table includes all items of   |
 |                                                                    | another, irrespective of their keys.          |
 +--------------------------------------------------------------------+-----------------------------------------------+
diff --git a/luatest/assertions.lua b/luatest/assertions.lua
index 842e705..693eafa 100644
--- a/luatest/assertions.lua
+++ b/luatest/assertions.lua
@@ -411,6 +411,21 @@ function M.assert_items_include(actual, expected, message)
     end
 end
 
+--- Checks that one table does not include any items of another, irrespective of their keys.
+--
+-- @param actual
+-- @param expected
+-- @string[opt] message
+function M.assert_items_exclude(actual, expected, message)
+    if type(actual) ~= 'table' or type(expected) ~= 'table' then
+        failure('Argument 1 and 2 must be tables', nil, 2)
+    end
+    if not comparator.are_disjoint(expected, actual) then
+        expected, actual = prettystr_pairs(expected, actual)
+        fail_fmt(2, message, 'Expected no item values from: %s\nTo be present in: %s', expected, actual)
+    end
+end
+
 local function table_slice(actual, expected)
     if type(expected) ~= 'table' or type(actual) ~= 'table' then
         return actual
diff --git a/luatest/comparator.lua b/luatest/comparator.lua
index 3377b45..699eee3 100644
--- a/luatest/comparator.lua
+++ b/luatest/comparator.lua
@@ -70,6 +70,23 @@ function comparator.is_subset(actual, expected)
     return #expected_array - found_count
 end
 
+-- Returns false if 'actual' or 'expected' are not tables or their value sets
+-- intersect. Returns true otherwise.
+function comparator.are_disjoint(actual, expected)
+    if (type(actual) ~= 'table') or (type(expected) ~= 'table') then
+        return false
+    end
+
+    for _, a in pairs(actual) do
+        for _, b in pairs(expected) do
+            if comparator.equals(a, b) then
+                return false
+            end
+        end
+    end
+    return true
+end
+
 -- This is a specialized metatable to help with the bookkeeping of recursions
 -- in table_equals(). It provides an __index table that implements utility
 -- functions for easier management of the table. The "cached" method queries
diff --git a/test/luaunit/assertions_test.lua b/test/luaunit/assertions_test.lua
index bdc1705..c82acb1 100644
--- a/test/luaunit/assertions_test.lua
+++ b/test/luaunit/assertions_test.lua
@@ -333,6 +333,39 @@ function g.test_assert_items_include()
     assert_failure(subject, {1,2,3}, {1,1,2,3})
 end
 
+function g.test_assert_items_exclude()
+    local subject = t.assert_items_exclude
+    assert_failure(subject, {1,2,3}, {3,1,2})
+    assert_failure(subject, {one=1,two=2,three=3}, {two=2,one=1,three=3})
+    assert_failure(subject, {one=1,two=2,three=3}, {a=1,b=2,c=3})
+    assert_failure(subject, {1,2,three=3}, {3,1,two=2})
+
+    assert_failure(subject, {1,2,3,4}, {3,1,2})
+    assert_failure(subject, {1,1,2,3}, {3,1,2})
+    assert_failure(subject, {1,2,3}, {1,2,3,4})
+    assert_failure(subject, {1,2,3}, {1,1,2,3})
+
+    assert_failure(subject, nil, {1})
+    assert_failure(subject, {}, nil)
+    assert_failure(subject, 1, {1})
+    assert_failure(subject, {}, 1)
+    assert_failure(subject, 'asd', {one = 'asd', two = 'dsa', three = 1})
+    assert_failure(subject, {one = 'asd', two = 'dsa', three = 1}, 'dsa')
+    assert_failure(subject, 1, 1)
+    assert_failure(subject, nil, 1)
+    assert_failure(subject, 1, nil)
+
+    subject({}, {1})
+    subject({},{})
+    subject({nil},{nil})
+    subject({1},{})
+    subject({one=1},{})
+    subject({},{one=1})
+    subject({1, 2, 3},{4, 5, 6, 7})
+    subject({one=1, two=2, three=3},{four=4, five=5})
+    subject({one=1, 2, 3},{four=4, 5})
+end
+
 function g.test_assert_nan()
     assert_failure(t.assert_nan, "hi there!")
     assert_failure(t.assert_nan, nil)