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initialize_ot2_simulate.py
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import custom_OT2_functions_simulate as ot2
#import custom_OT2_functions as ot2
import math
import json
import time
import opentrons.execute
from opentrons import protocol_api
################################
##### INITIALIZE LIBRARIES #####
################################
# Initialize library to use Protocol API version on robot.
# DO NOT CHANGE UNLESS THE ROBOT SOFTWARE IS UPDATED!
protocol = opentrons.execute.get_protocol_api('2.7')
metadata = {'apiLevel': '2.7'}
####################################
##### INITIALIZE ROBOT MODULES #####
####################################
# Load the custom labware definitions
#with open('brautech_8_wellplate_20000ul.json') as labware_file:
# stocks_labware_def = json.load(labware_file)
#stocks_labware_def = json.load(open('brautech_8_wellplate_20000ul.json'))
#with open('agilent_54_wellplate_2000ul.json') as labware_file:
# samples_labware_def = json.load(labware_file)
#samples_labware_def = json.load(open('agilent_54_wellplate_2000ul.json'))
# Load pipette(s) and tiprack(s)
pipette_large = {
"protocol": protocol.load_instrument('p300_single_v1.5', mount='left'),
"tiprack": protocol.load_labware('opentrons_96_tiprack_300ul', 10),
"model": "P300",
"maxVolume": 300,
"minVolume": 30,
"aspirateVolume": 250,
"tipNumber": 0
}
pipette_small = {
"protocol": protocol.load_instrument('p10_single_v1.5', mount='right'),
"tiprack": protocol.load_labware('opentrons_96_tiprack_10ul', 11),
"model": "P10",
"maxVolume": 10,
"minVolume": 1,
"aspirateVolume": 10,
"tipNumber": 0
}
# Load the labware that holds the 50 mL solvent vials
#solvents = protocol.load_labware('opentrons_6_tuberack_nest_50ml_conical', 1)
# Load the labware that hold the 20 mL stock vials
#stocks = protocol.load_labware_from_definition(stocks_labware_def, 2)
# Load the labware that hold the 2 mL sample vials
#samples = protocol.load_labware_from_definition(samples_labware_def, 3)
def getTipLocation(tipNumber):
rowNum = math.floor(tipNumber / 12)
# print(rowNum)
columnNum = (tipNumber % 12) + 1
rowLetters = ("A","B","C","D","E","F","G","H")
return rowLetters[rowNum] + str(columnNum)
def aspirateVolume(volumeTotal, source_plate, source_location, source_volume, source_vialType, dest_plate, dest_location, dest_volume_total, newPipetteTip = 1, dispenseRate = 1):
dest_volume = 0
if source_volume - volumeTotal < 0:
raise Exception("Source vial volume is too low!")
pipette_large_hasTip = 0
pipette_small_hasTip = 0
if math.floor(volumeTotal / pipette_large["aspirateVolume"]) > 0 and newPipetteTip == 1:
ot2.PickUpTip(pipette_large["protocol"], pipette_large["tiprack"], getTipLocation(pipette_large["tipNumber"]))
pipette_large["tipNumber"] += 1
pipette_large_hasTip = 1
for i in range(math.floor(volumeTotal / pipette_large["aspirateVolume"])):
# dest_volume += pipette_large["aspirateVolume"]
source_volume = ot2.aspirateVolume(pipette_large["protocol"], source_plate, source_location, source_vialType, source_volume, pipette_large["aspirateVolume"])
dest_volume = ot2.dispenseVolume(pipette_large["protocol"], dest_plate, dest_location, dest_volume, pipette_large["aspirateVolume"], dispenseRate)
print(" sample volume = " + str(dest_volume) + " uL (+" + str(pipette_large["aspirateVolume"]) + " uL from " + pipette_large["model"] + ")")
volumeRemaining = volumeTotal - dest_volume
#print(" remaining = " + str(volumeRemaining) + " uL")
if volumeRemaining == 0:
if pipette_large_hasTip == 1 and newPipetteTip == 1:
ot2.DropTip(pipette_large["protocol"])
return source_volume, (dest_volume + dest_volume_total), volumeRemaining
if volumeRemaining >= pipette_large["minVolume"]:
if pipette_large_hasTip == 0 and newPipetteTip == 1:
ot2.PickUpTip(pipette_large["protocol"], pipette_large["tiprack"], getTipLocation(pipette_large["tipNumber"]))
pipette_large["tipNumber"] += 1
pipette_large_hasTip = 1
# dest_volume += volumeRemaining
source_volume = ot2.aspirateVolume(pipette_large["protocol"], source_plate, source_location, source_vialType, source_volume, volumeRemaining)
dest_volume = ot2.dispenseVolume(pipette_large["protocol"], dest_plate, dest_location, dest_volume, volumeRemaining, dispenseRate)
print(" sample volume = " + str(dest_volume) + " uL (+" + str(volumeRemaining) + " uL from " + pipette_large["model"] + ")")
if pipette_large_hasTip == 1 and newPipetteTip == 1:
ot2.DropTip(pipette_large["protocol"])
if volumeRemaining < pipette_large["minVolume"]:
if math.floor(volumeRemaining / pipette_small["aspirateVolume"]) > 0 and newPipetteTip == 1:
ot2.PickUpTip(pipette_small["protocol"], pipette_small["tiprack"], getTipLocation(pipette_small["tipNumber"]))
pipette_small["tipNumber"] += 1
pipette_small_hasTip = 1
for i in range(math.floor(volumeRemaining / pipette_small["aspirateVolume"])):
# dest_volume += pipette_small["aspirateVolume"]
source_volume = ot2.aspirateVolume(pipette_small["protocol"], source_plate, source_location, source_vialType, source_volume, pipette_small["aspirateVolume"])
dest_volume = ot2.dispenseVolume(pipette_small["protocol"], dest_plate, dest_location, dest_volume, pipette_small["aspirateVolume"], dispenseRate)
print(" sample volume = " + str(dest_volume) + " uL (+" + str(pipette_small["aspirateVolume"]) + " uL from " + pipette_small["model"] + ")")
volumeRemaining = volumeTotal - dest_volume
#print(" remaining = " + str(volumeRemaining) + " uL")
if volumeRemaining == 0:
if pipette_small_hasTip == 1 and newPipetteTip == 1:
ot2.DropTip(pipette_small["protocol"])
return source_volume, (dest_volume + dest_volume_total), volumeRemaining
if volumeRemaining >= pipette_small["minVolume"]:
if pipette_small_hasTip == 0 and newPipetteTip == 1:
ot2.PickUpTip(pipette_small["protocol"], pipette_small["tiprack"], getTipLocation(pipette_small["tipNumber"]))
pipette_small["tipNumber"] += 1
pipette_small_hasTip = 1
# dest_volume += volumeRemaining
source_volume = ot2.aspirateVolume(pipette_small["protocol"], source_plate, source_location, source_vialType, source_volume, volumeRemaining)
dest_volume = ot2.dispenseVolume(pipette_small["protocol"], dest_plate, dest_location, dest_volume, volumeRemaining, dispenseRate)
print(" sample volume = " + str(dest_volume) + " uL (+" + str(volumeRemaining) + " uL from " + pipette_small["model"] + ")")
if pipette_small_hasTip == 1 and newPipetteTip == 1:
ot2.DropTip(pipette_small["protocol"])
volumeRemaining = volumeTotal - dest_volume
#print(" FINAL remaining = " + str(volumeRemaining) + " uL")
#print("")
return source_volume, (dest_volume + dest_volume_total), volumeRemaining
ot2.ResetRobot()