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Rabies genome annotation #707

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marco315176 opened this issue Sep 3, 2024 · 0 comments
Open

Rabies genome annotation #707

marco315176 opened this issue Sep 3, 2024 · 0 comments

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@marco315176
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Hi, I have a problem annotating genome of my rabies samples. Although I have a file with gene specific proteins in .faa format, at the time of annotation it marks me that some are either smaller or larger. The code I am using is the following, I don't know if I am making a mistake.
Thank you very much in advance.

for assembly in /home/secuenciacion_cenasa/Analisis_corridas/SPAdes_viral/*.fa; do
ID=$(basename ${assembly} | cut -d '-' -f '1')
ename=$(basename ${assembly} | cut -d '.' -f '1')

prokka ${assembly}
--proteins /home/secuenciacion_cenasa/Programas_Bioinformaticos/prokka/db/genus/Rabia_prokka_db/tmp/Rabies_proteins.faa
--outdir /home/secuenciacion_cenasa/Analisis_corridas/Resultados_all_virus/prokka/${ID}_${gene}_anotado
--evalue 1e-9
--coverage 100
--rawproduct

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