diff --git a/lib/galaxy/config/sample/datatypes_conf.xml.sample b/lib/galaxy/config/sample/datatypes_conf.xml.sample
index f97bf1f54c73..e2d80eae608d 100644
--- a/lib/galaxy/config/sample/datatypes_conf.xml.sample
+++ b/lib/galaxy/config/sample/datatypes_conf.xml.sample
@@ -210,6 +210,9 @@
       <display file="igv/interval_as_bed.xml" inherit="true"/>
     </datatype>
     <datatype extension="jellyfish" type="galaxy.datatypes.binary:Binary" subclass="true" display_in_upload="true" description="Jellyfish database files are k-mer counts in binary format with a readable head. They are operated on and converted to human-readable text through jellyfish commands." />
+    <datatype extension="ktab" type="galaxy.datatypes.binary:Binary" subclass="true" description="A table of canonical k‑mers and their counts for the fastk toolkit." display_in_upload="true" description_url="https://github.com/thegenemyers/FASTK?tab=readme-ov-file#file-encodings"/>
+    <datatype extension="hist" type="galaxy.datatypes.binary:Binary" subclass="true" description="A binary histogram file of kmers and frequencies for the fastk toolkit." display_in_upload="true" description_url="https://github.com/thegenemyers/FASTK?tab=readme-ov-file#file-encodings"/>
+    <datatype extension="prof" type="galaxy.datatypes.binary:Binary" subclass="true" description="Read profile file for the fastk toolkit." display_in_upload="true" description_url="https://github.com/thegenemyers/FASTK?tab=readme-ov-file#file-encodings"/>
 
     <!-- ISA data types -->
     <datatype extension="isa-tab" type="galaxy.datatypes.isa:IsaTab" mimetype="application/isa-tools" display_in_upload="true" description="ISA-Tab data type." description_url="https://isa-tools.org"/>