From 5e91e59731cc83ff549f3c3e6ec3de7fbd0aea3f Mon Sep 17 00:00:00 2001 From: Tom Harrop Date: Wed, 6 Nov 2024 14:29:04 +1100 Subject: [PATCH 1/4] adjust logic for custom primers --- tools/dorado/dorado_trimming.xml | 28 ++++++++++++------ .../test-data/dorado_trimming_test1.bam | Bin 60520 -> 60725 bytes .../test-data/dorado_trimming_test1.tsv | 10 +++---- .../test-data/dorado_trimming_test2.bam | Bin 60621 -> 60628 bytes .../test-data/dorado_trimming_test2.tsv | 8 ++--- .../test-data/dorado_trimming_test3.bam | Bin 787 -> 798 bytes 6 files changed, 28 insertions(+), 18 deletions(-) diff --git a/tools/dorado/dorado_trimming.xml b/tools/dorado/dorado_trimming.xml index d4c1c778..3c8d2c47 100644 --- a/tools/dorado/dorado_trimming.xml +++ b/tools/dorado/dorado_trimming.xml @@ -14,11 +14,12 @@ ln -s '$reads' ./reads dorado trim --verbose --threads "\${GALAXY_SLOTS}" -#if $no_trim_primers +#if $trim_primers.no_trim_primers == 'dont_trim' --no-trim-primers -#end if -#if $primer_sequences - --primer-sequences '$primer_sequences' +#else + #if $trim_primers.primer_sequences + --primer-sequences '${trim_primers.primer_sequences}' + #end if #end if reads > trimmed.bam @@ -33,8 +34,16 @@ trimmed.bam ]]> - - + + + + + + + + + + @@ -48,7 +57,7 @@ trimmed.bam - + @@ -75,8 +84,9 @@ adapters and primers may result in some portions of the flanking regions of the barcodes being removed, which could interfere with correct demultiplexing. -The **Don't trim primers** option can be used to prevent the trimming of -primer sequences. In this case only adapter sequences will be trimmed. +The **Enable primer trimming** option can be set to 'No' to prevent the +trimming of primer sequences. If you select 'No', only adapter sequences will +be trimmed. The output of will always be unaligned records, regardless of whether the input is aligned/sorted or not. diff --git a/tools/dorado/test-data/dorado_trimming_test1.bam b/tools/dorado/test-data/dorado_trimming_test1.bam index 5909004217915c08e3f88807a9f5abd39bd9cffd..827d7f5b628b8e73bf245c744897e94bac80559f 100644 GIT binary patch literal 60725 zcmV)5K*_%!iwFb&00000{{{d;LjnLb0gcd0Zo)7S24Gm4Q)G`~#&MpOkU|>uAtewM z+HGWGM@0>GQGlxQau>>1%mWWxh+X2ZfG`p>0vG_s01Nf z8xT+=2n<&eW*`ithJZ$9Wd>A>E@?V@8nciVBq)JvZM2<{Fk3AJXsxAwfj0U95+L+e z&|>s`MlaP&S8dxP!o&SuUh*6}W}kg#$7uZ4{ph2A`}XzmN3;IuBkQ9V>-VpHgs;y& zd;k9ZufO`?(_jDa<2Rpv_5J(rKK=Hauik(9!w)}w{o(5mUw-)VhYufZ{aSDJ+H}i4 zZu6q*y&qIvFI&CrYOU*aQ=6&xWnA`p-u9uc)wWMPzYqJSmb%$@HLHDDv|5&Z#|N~= z8?~zO=|<@t$1Zwr=K*KH=~so*_w%~eG0?=!}R6RB;PAq%C{JPiB3D1O_PS`9d8#LH?}RKwHoG74j*jP_&N zcxl165x-&HJZR?;JI2L#@#~_EQaqi4bmR!kc;9e+oCCis13$t+F8t5%#|A%b{oXFP z#)N;6K14ua^ok>Z*kxk4hJmd1ZJv3(Wm9|VzOdx7r zTHmVs<92(-!Nv2{B<?_xs~|sh=;^rK;*Y&ua`IPh2ku+*B7TjIQ^})YseNk;akh 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+1,11 @@ filename read_id run_id channel mux start_time duration template_start template_duration sequence_length_template mean_qscore_template barcode reads.pod5 00777c4b-cbd6-4a79-8647-bbe5f5f3f3bf 473bf0edfc2f8f756173de35db5da9b6f6db4959 300 3 329.733 2.5165 329.766 2.484 1055 12.9149 unclassified +reads.pod5 002f231b-5d37-437f-a027-a2e8b872e73b 473bf0edfc2f8f756173de35db5da9b6f6db4959 118 3 534.745 19.5847 534.745 19.5847 8387 9.88254 unclassified reads.pod5 0072b26f-f37c-4517-afa7-621543ac2187 473bf0edfc2f8f756173de35db5da9b6f6db4959 317 3 111.275 2.31875 111.275 2.31875 929 9.69614 unclassified reads.pod5 0067486b-9f92-4849-8456-671463e64412 473bf0edfc2f8f756173de35db5da9b6f6db4959 84 4 164.018 2.453 164.018 2.453 885 12.399 unclassified -reads.pod5 002f231b-5d37-437f-a027-a2e8b872e73b 473bf0edfc2f8f756173de35db5da9b6f6db4959 118 3 534.745 19.5847 534.745 19.5847 8387 9.88254 unclassified +reads.pod5 000a9728-0a7c-4b64-9791-76bb30b63796 473bf0edfc2f8f756173de35db5da9b6f6db4959 105 4 331.319 3.27625 331.319 3.27625 1175 12.0328 unclassified +reads.pod5 005cf7ae-4d74-42dd-ab96-9befed842822 473bf0edfc2f8f756173de35db5da9b6f6db4959 473 2 258.872 29.436 259.165 29.1435 14587 5.17895 unclassified reads.pod5 0066800d-d191-4833-a495-cfe8b925aca0 473bf0edfc2f8f756173de35db5da9b6f6db4959 56 4 359.11 14.0378 359.11 14.0378 5111 9.1072 unclassified reads.pod5 009f5efd-de5d-4a7e-9d17-969c3996cbc8 473bf0edfc2f8f756173de35db5da9b6f6db4959 230 2 171.634 3.27475 171.634 3.27475 1232 13.6258 unclassified -reads.pod5 005cf7ae-4d74-42dd-ab96-9befed842822 473bf0edfc2f8f756173de35db5da9b6f6db4959 473 2 258.872 29.436 259.165 29.1435 14587 5.17895 unclassified -reads.pod5 000a9728-0a7c-4b64-9791-76bb30b63796 473bf0edfc2f8f756173de35db5da9b6f6db4959 105 4 331.319 3.27625 331.319 3.27625 1175 12.0328 unclassified -reads.pod5 00ad521b-b916-404f-a31d-a657f9aa7756 473bf0edfc2f8f756173de35db5da9b6f6db4959 365 2 566.263 23.6745 566.263 23.6745 10934 12.074 unclassified reads.pod5 00aeb4ba-e404-49d2-97c5-8fcf22547f81 473bf0edfc2f8f756173de35db5da9b6f6db4959 51 4 297.891 50.1888 297.984 50.0962 19142 8.45178 unclassified +reads.pod5 00ad521b-b916-404f-a31d-a657f9aa7756 473bf0edfc2f8f756173de35db5da9b6f6db4959 365 2 566.263 23.6745 566.263 23.6745 10934 12.074 unclassified diff --git a/tools/dorado/test-data/dorado_trimming_test3.bam b/tools/dorado/test-data/dorado_trimming_test3.bam index 32bf1d097f4412dbddc5c9ed053fa3bc6094e67d..8329cbe160368d077bad5acb717820a74fb803b4 100644 GIT binary patch delta 223 zcmV<503iR92A&3gABzYC000000RIL6LPG)o*8nY#&1%9x9K_>seTu!yeq^&XCMT;x z6HZZReHkCXUk;6^o^od0{HFef_WSL}Ef#2JpHIkX-!oplraoNa3DZ z@Fc)@53PZKS-jmRjpOK=#t+P0t`L}DoLOd7Q~}~ya9dTbDu`00B+?m4v{WTAh0vvO Z{7LhiCK&%M&C{9e7iTau$=0#TNdmdJY&rk{ delta 212 zcmV;_04x8V29pMVABzYC000000RIL6LPG)o%m6Kq$qK?S7=-IFeTv*AO_SEv=HyaP z!KEO0l`Ov~YFnEWU(ZJ~6%TWmnGc3p%y*+t5CpSziB5aLq6{4mqOb3D^RB~LwL+Ux zxbF5u)n1sUlcpKCIGRR$oT-#0x$2-TMGS! O;0shFN7>A=)ky+ZdS8_Q From 911f2af3b58dbf4bc3b0fba0afa500b161137f73 Mon Sep 17 00:00:00 2001 From: Tom Harrop Date: Wed, 6 Nov 2024 14:29:22 +1100 Subject: [PATCH 2/4] version --- tools/dorado/dorado_trimming.xml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/tools/dorado/dorado_trimming.xml b/tools/dorado/dorado_trimming.xml index 3c8d2c47..b0a94b07 100644 --- a/tools/dorado/dorado_trimming.xml +++ b/tools/dorado/dorado_trimming.xml @@ -1,4 +1,4 @@ - + for Oxford Nanopore (ONT) DNA reads macros.xml From f1c9784a23795180211e2cc0623a0c2089d605a0 Mon Sep 17 00:00:00 2001 From: Tom Harrop Date: Wed, 6 Nov 2024 14:57:26 +1100 Subject: [PATCH 3/4] fix tests --- tools/dorado/dorado_trimming.xml | 36 +++++++++++++++--- .../test-data/dorado_trimming_test1.bam | Bin 60725 -> 0 bytes .../test-data/dorado_trimming_test1.tsv | 11 ------ .../test-data/dorado_trimming_test2.bam | Bin 60628 -> 0 bytes .../test-data/dorado_trimming_test2.tsv | 11 ------ .../test-data/dorado_trimming_test3.bam | Bin 798 -> 0 bytes .../test-data/dorado_trimming_test3.tsv | 2 - 7 files changed, 30 insertions(+), 30 deletions(-) delete mode 100644 tools/dorado/test-data/dorado_trimming_test1.bam delete mode 100644 tools/dorado/test-data/dorado_trimming_test1.tsv delete mode 100644 tools/dorado/test-data/dorado_trimming_test2.bam delete mode 100644 tools/dorado/test-data/dorado_trimming_test2.tsv delete mode 100644 tools/dorado/test-data/dorado_trimming_test3.bam delete mode 100644 tools/dorado/test-data/dorado_trimming_test3.tsv diff --git a/tools/dorado/dorado_trimming.xml b/tools/dorado/dorado_trimming.xml index b0a94b07..558c31b8 100644 --- a/tools/dorado/dorado_trimming.xml +++ b/tools/dorado/dorado_trimming.xml @@ -52,20 +52,44 @@ trimmed.bam - - + + + + + + + + + + - - + + + + + + + + + + - - + + + + + + + + + + uAtewM z+HGWGM@0>GQGlxQau>>1%mWWxh+X2ZfG`p>0vG_s01Nf z8xT+=2n<&eW*`ithJZ$9Wd>A>E@?V@8nciVBq)JvZM2<{Fk3AJXsxAwfj0U95+L+e 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