diff --git a/.ci/check_files.py b/.ci/check_files.py
index e7a32e061..3dcd18ebb 100644
--- a/.ci/check_files.py
+++ b/.ci/check_files.py
@@ -29,7 +29,7 @@
     'ChemicalToolBox', 'Qualimap', 'Viral Tools', 'Single-cell', 'RAD-seq',
     'None', 'Epigenetics', 'HiCExplorer', 'Imaging', 'DNA Metabarcoding',
     'Other Tools', 'Species abundance', 'Climate Analysis', 'HCA Single-cell',
-    'MiModD', 'BB Tools', 'Proteomic AI'
+    'MiModD', 'BB Tools', 'Proteomic AI', 'pRESTO',
 ]
 
 
diff --git a/requests/presto.yml b/requests/presto.yml
new file mode 100644
index 000000000..31c8c76e3
--- /dev/null
+++ b/requests/presto.yml
@@ -0,0 +1,46 @@
+tools:
+- name: presto_alignsets
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_assemblepairs
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_buildconsensus
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_collapseseq
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_filterseq
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_maskprimers
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_pairseq
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_parseheaders
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_parselog
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: presto_partition
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+- name: prestor_abseq3
+  owner: iuc
+  tool_panel_section_label: pRESTO
+  tool_shed_url: toolshed.g2.bx.psu.edu
+