From cb09c5c9025f34d37ab3cacd8628985260f6db09 Mon Sep 17 00:00:00 2001
From: Laura190 <Laura190@users.noreply.github.com>
Date: Tue, 23 Apr 2024 14:52:09 +0100
Subject: [PATCH] Add files via upload

---
 CountSpotsInCells.ijm | 4 ++--
 1 file changed, 2 insertions(+), 2 deletions(-)

diff --git a/CountSpotsInCells.ijm b/CountSpotsInCells.ijm
index 50ae98a..aa18d69 100644
--- a/CountSpotsInCells.ijm
+++ b/CountSpotsInCells.ijm
@@ -63,10 +63,10 @@ selectImage(original);
 original=getTitle();
 Stack.setPosition(1, 1, 1);
 run("Duplicate...", "title=copy duplicate channels=1");
-run("Median...", "radius=1");
+run("Median...", "radius=2");
 roiManager("Deselect");
 roiManager("Combine");
-run("Find Maxima...", "prominence=140 output=[Point Selection]");
+run("Find Maxima...", "prominence=200 output=[Point Selection]");
 selectImage(original);
 run("Restore Selection");
 roiManager("add");