diff --git a/shadow-dom/reference-target/tentative/interesttarget.tentative.html b/shadow-dom/reference-target/tentative/interesttarget.tentative.html
new file mode 100644
index 00000000000000..e8d7c814d865fa
--- /dev/null
+++ b/shadow-dom/reference-target/tentative/interesttarget.tentative.html
@@ -0,0 +1,67 @@
+<!DOCTYPE HTML>
+<html>
+
+<head>
+  <script src="/resources/testharness.js"></script>
+  <script src="/resources/testharnessreport.js"></script>
+  <script src="/resources/testdriver.js"></script>
+  <script src="/resources/testdriver-vendor.js"></script>
+  <script src="/resources/testdriver-actions.js"></script>
+  <script src="/wai-aria/scripts/aria-utils.js"></script>
+  <script src="/html/semantics/the-button-element/interest-target/resources/invoker-utils.js"></script>
+</head>
+
+<body>
+  <style>
+      [interesttarget] {
+          interest-target-delay: 0s;
+      }
+  </style>
+  <button id="toggle-button-1" interesttarget="x-popover-1">Toggle the popover</button>
+  <x-popover-1 id="x-popover-1">
+    <template shadowrootmode="open" shadowrootreferencetarget="popover">
+      <div id="popover" popover>Popover content inside shadow root</div>
+    </template>
+  </x-popover-1>
+
+  <button id="toggle-button-2" interesttarget="x-popover-2">Toggle the popover</button>
+  <x-popover-2 id="x-popover-2"></x-popover-2>
+  <script>
+    const customPopover2 = document.querySelector('x-popover-2');
+    customPopover2.attachShadow({ mode: 'open', referenceTarget: 'popover' });
+    customPopover2.shadowRoot.innerHTML = '<div id="popover" popover>Popover content inside shadow root</div>';
+  </script>
+
+  <button id="toggle-button-3">Toggle the popover</button>
+  <x-popover-3 id="x-popover-3">
+    <template shadowrootmode="open" shadowrootreferencetarget="popover">
+      <div id="popover" popover>Popover content inside shadow root</div>
+    </template>
+  </x-popover-3>
+
+  <script>
+    function testInterestTarget(popoverId, buttonId, name) {
+        promise_test(async function () {
+        const xPopover = document.getElementById(popoverId);
+        const popover = xPopover.shadowRoot.getElementById("popover");
+
+        let showCount = 0;
+        popover.addEventListener('beforetoggle', (e) => {
+          if (e.newState === "open")
+            ++showCount;
+        });
+
+        const toggleButton = document.getElementById(buttonId);
+        await hoverOver(toggleButton);
+
+        assert_equals(showCount, 1, "showCount");
+      }, name);
+    }
+    testInterestTarget('x-popover-1', 'toggle-button-1', "Shadow root reference target works with interesttarget attribute.");
+    testInterestTarget('x-popover-2', 'toggle-button-2', "Shadow root reference target works with interesttarget attribute via options.");
+    document.getElementById('toggle-button-3').interestTargetElement = document.getElementById('x-popover-3');
+    testInterestTarget('x-popover-3', 'toggle-button-3', "Shadow root reference target works with .interestTargetElement property.");
+  </script>
+</body>
+
+</html>
