annotation files for hg19

[yangjywhu]

Hello!
We have annotation files of hg38 and mm10.
But I need to run PAQR in our data, which has been mapped to hg19.
Could you provide the annotation files of hg19? or, can I change the coordinate from hg38 to hg19 in the provided files?

Best wishes,
Jiayi Yang

[koljaLanger]

Hi Jiayi Yang

unfortunately, we can not provide annotation files for hg19 at the moment.

However, we might be able to create them for you once you told us which annotation you used for the mapping. It is important to have in mind that not only the genome build version makes a difference but also whether the annotation is provided by ENSEMBL or NCBI (most of the time).

Moreover, we would filter more restrictive if your RNA-seq data is unstranded. Therefore, it would be best to have this information as well.

Hopefully, this would help you,
best regards,

Ralf