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实验记录 2023-06-26 #21

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zemise opened this issue Jun 26, 2023 · 0 comments
Open

实验记录 2023-06-26 #21

zemise opened this issue Jun 26, 2023 · 0 comments

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@zemise
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zemise commented Jun 26, 2023

2023-06-26

周一

第一次做梦笑醒

梦中大致是多队人比赛渡河,规则是一个人踩着其他队员的背部渡河,似乎掉下河就算输,并且不知道队伍中的谁是踩背者。每个队伍的踩背者都很谨小慎微,唯独有只队伍的踩背者,出发即冲刺,早早掉下河。那只队伍的后半段成员在讨论是哪个二货出发即冲刺,后续发生了一些搞笑的事情,可惜到这就记忆模糊了

Mission 1

Western blot

2023-06-25 后续显影结果

样本
5-8F/A549/Caski + NEes (0, 1, 5, 10, 20, 40) 8h

b-actin+Marker_PUB_300
capase-3-浅+Marker_PUB_300
PARP-浅+Marker_PUB_300

Mission 2

细胞处理

细胞株 培基 处理
A549 DMEM(10%FBS + 1% Pen/Strep) 细胞偏少,仅换液
5-8F DMEM(10%FBS + 1% Pen/Strep) 细胞偏少,仅换液
HUVEC DMEM(10%FBS + 1% Pen/Strep) 换液,并种24孔板,6孔

IMG_1213

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