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两套单倍型分配不平均 #34

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1203450402 opened this issue Aug 5, 2024 · 2 comments
Open

两套单倍型分配不平均 #34

1203450402 opened this issue Aug 5, 2024 · 2 comments

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@1203450402
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张老师好! 我组装了一个2倍体基因组, 因为做kmer分析的时候, 整个基因组的杂合度很高, 所以选择装出两套单倍型基因组,我想用subphaser来对这两套单倍型基因组进行分型, -f 参数默认是2,如果我使用-f 默认参数就显示ValueError: 0 kmer remained after filtering. Please reset the filter options. 后来我将-f参数设为1.6, 跑出来的结果发现染色体分配不平均, 一组为10条,一组为16条。这种情况应该怎样处理,能使得染色体平均分组?
我的代码: subphaser -i wzmt.fasta -c sp.config -disable_ltr -f 1.6 -pre sp_out > sp.log

sp_outk15_q200_f1.6.circos.pdf
sp_outk15_q200_f1.6.kmer.mat.pdf
sp_outk15_q200_f1.6.kmer_freq.pdf
sp_outk15_q200_f1.6.kmer_pca.pdf

@zhangrengang
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zhangrengang commented Aug 5, 2024 via email

@1203450402
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好的,谢谢老师!

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