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I do my phastCons search for rho and then I re-execute phastCons to determine elements. I then filter regions e use to extract those regions with hal2maf again, to have a look at them.
They seem to be full of gaps. I don't know how familiar you are with such analysis, but I was wondering if I'm doing something wrong with my conversion. Do I have to sort the maf files or convert it into fastas, and how could I do that?
Thanks a lot
best
F
The text was updated successfully, but these errors were encountered:
Hi @glennhickey,
I'm trying to run a PhastCons analysis in order to identify conserved elements.
I'm converting each chromosome with the following command:
hal2maf --refGenome $SP --noAncestors --noDupes --onlyOrthologs --refSequence $scf $HAL $scf.maf
I do my phastCons search for rho and then I re-execute phastCons to determine elements. I then filter regions e use to extract those regions with
hal2maf
again, to have a look at them.They seem to be full of gaps. I don't know how familiar you are with such analysis, but I was wondering if I'm doing something wrong with my conversion. Do I have to sort the maf files or convert it into fastas, and how could I do that?
Thanks a lot
best
F
The text was updated successfully, but these errors were encountered: