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Merge pull request galaxyproject#372 from debjyoti197/qiime2-part2
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QIIME2 downstream workflows
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mvdbeek authored Aug 6, 2024
2 parents ae9ede1 + 0914c96 commit 2b350d7
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25 changes: 25 additions & 0 deletions workflows/amplicon/qiime2/qiime2-III-VI-downsteam/.dockstore.yml
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version: 1.2
workflows:
- name: QIIME2-III-V-Phylogeny-Rarefaction-Taxonomic-Analysis
subclass: Galaxy
publish: true
primaryDescriptorPath: /QIIME2-III-V-Phylogeny-Rarefaction-Taxonomic-Analysis.ga
testParameterFiles:
- /QIIME2-III-V-Phylogeny-Rarefaction-Taxonomic-Analysis-tests.yml
authors:
- name: Debjyoti Ghosh
orcid: 0009-0008-1496-1677
- name: "Helmholtz-Zentrum für Umweltforschung - UFZ"
address: "Permoserstraße 15, 04318 Leipzig"

- name: QIIME2-VI-diversity-metrics-and-estimations
subclass: Galaxy
publish: true
primaryDescriptorPath: /QIIME2-VI-diversity-metrics-and-estimations.ga
testParameterFiles:
- /QIIME2-VI-diversity-metrics-and-estimations-tests.yml
authors:
- name: Debjyoti Ghosh
orcid: 0009-0008-1496-1677
- name: "Helmholtz-Zentrum für Umweltforschung - UFZ"
address: "Permoserstraße 15, 04318 Leipzig"
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version: '0.1'
registries:
- url: https://workflowhub.eu
project: iwc
workflow: qiime2/main
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# Changelog

## [0.1] 2024-06-22
First release.
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- doc: Test outline for QIIME2-III:-Phylogenetic-tree-for-diversity-analysis
job:
Metadata:
class: File
location: https://data.qiime2.org/2024.2/tutorials/pd-mice/sample_metadata.tsv
filetype: tabular
Representative sequences:
class: File
location: https://docs.qiime2.org/2024.2/data/tutorials/pd-mice/dada2_rep_set.qza
filetype: qza
Feature table:
class: File
location: https://docs.qiime2.org/2024.2/data/tutorials/pd-mice/dada2_table.qza
filetype: qza
Minimum depth: 1
Maximum depth: 2019
Taxonomic classifier:
class: File
location: https://docs.qiime2.org/2024.2/data/tutorials/pd-mice/gg-13-8-99-515-806-nb-classifier.qza
filetype: qza
SEPP fragment insertion reference:
class: File
location: https://data.qiime2.org/2024.2/common/sepp-refs-gg-13-8.qza
filetype: qza
outputs:
Rooted tree:
ftype: qza
asserts:
- has_size:
min: 2M
max: 3M
- has_archive_member:
path: "^[^/]*/data/tree.nwk"
n: 1
asserts:
- has_text_matching:
expression: "k__Bacteria"
- has_archive_member:
path: "^[^/]*/metadata.yaml"
n: 1
asserts:
- has_line:
line: "type: Phylogeny[Rooted]"
- has_line:
line: "format: NewickDirectoryFormat"

Rarefaction curve:
ftype: qzv
asserts:
- has_size:
min: 400k
max: 500k
- has_archive_member:
path: "^[^/]*/data/index.html"
n: 1
# data per measure
- has_archive_member:
path: "^[^/]*/data/.*\\.csv"
n: 3
# data for each measure and metadata variable (*7)
- has_archive_member:
path: "^[^/]*/data/.*\\.jsonp"
n: 21

Taxonomy classification:
ftype: qza
asserts:
- has_size:
min: 70k
max: 80k
- has_archive_member:
path: "^[^/]*/metadata.yaml"
n: 1
asserts:
- has_line:
line: "type: FeatureData[Taxonomy]"
- has_line:
line: "format: TSVTaxonomyDirectoryFormat"
- has_archive_member:
path: "^[^/]*/data/taxonomy.tsv"
n: 1
asserts:
- has_n_lines:
n: 288
Taxa barplot:
ftype: qzv
asserts:
- has_size:
min: 400k
max: 500k
- has_archive_member:
path: "^[^/]*/data/.*\\.csv"
n: 7
- has_archive_member:
path: "^[^/]*/data/.*\\.jsonp"
n: 7
Taxonomy classification table:
ftype: qza
asserts:
- has_size:
min: 1M
max: 2M
- has_archive_member:
path: "^[^/]*/data/metadata.tsv"
n: 1
asserts:
- has_n_lines:
n: 289
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