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FelixKrueger committed Dec 9, 2024
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4 changes: 3 additions & 1 deletion docs/bismark/methylation_extraction.md
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Expand Up @@ -121,10 +121,12 @@ Both of these measures aim to reduce unwanted biases, i.e. the influence of `G-C

The `coverage2cytosine` module can be instructed to also extract a **f**our-, **f**ive- and **s**ix-nucleotide context (`--ffs`) for cytosines in question. Hexamers follow the rule `xxCxxx`. Too short sequences (e.g. at the edges of the chromosome) are left blank; sequences containing Ns are ignored.

```
Example:
U00096.3 90 + 0 0 CG CGT CGTG CGTGA GCCGTG
U00096.3 91 - 1 0 CG CGG CGGC CGGCA CACGGC

```

### M-bias plot

Starting with Bismark v0.8.0, the Bismark methylation extractor also produces a methylation bias plot which shows the methylation proportion across each possible position in the read (described in further detail in: [Hansen et al., Genome Biology, 2012, 13:R83](https://genomebiology.biomedcentral.com/articles/10.1186/gb-2012-13-10-r83)). The data for the M-bias plot is also written into a coverage text file (ending in `.cov` or `.cov.gz`) and is in the following format:
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4 changes: 3 additions & 1 deletion docs/options/methylation_extraction.md
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Expand Up @@ -163,10 +163,12 @@ The minimum number of times any methylation state (methylated or unmethylated) h

In addition to the standard output this option also extracts a **f**our-, **f**ive- and **s**ix-nucleotide context for the cytosines in question. Hexamers follow the rule `xxCxxx`. Too short sequences (e.g. at the edges of the chromosome) are left blank; sequences containing Ns are ignored. This option needs to be run via `coverage2cytosine` itself.

```
Example:
U00096.3 90 + 0 0 CG CGT CGTG CGTGA GCCGTG
U00096.3 91 - 1 0 CG CGG CGGC CGGCA CACGGC

```

#### OUTPUT

##### Extractor output
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