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FelixKrueger committed Oct 13, 2017
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## Changelog for Bismark v0.18.2_dev

### Bismark

Changed the methylation call behaviour so that insertions in a read (which are filled in with `X` for the methylation call) are also considered as `Unknown context` for the methylation call. Here is [issue#135](https://github.com/FelixKrueger/Bismark/issues/135).

### filter_non_conversion

Added new options `--percentage_cutoff [int]` and `--minimum_count [int]` to allow filtering reads for non-bisulfite conversion using an overall methylation percentage and count cutoff. Here is [issue #122](https://github.com/FelixKrueger/Bismark/issues/122).
Expand All @@ -10,14 +14,39 @@ Added new options `--percentage_cutoff [int]` and `--minimum_count [int]` to all

Added option `--multiple` to the deduplicator to treat several input SAM/BAM files as the same sample. Here is [issue #107](https://github.com/FelixKrueger/Bismark/issues/107).

Added option `-output_dir` to `deduplicate_bismark` so that it can be used in the Google cloud. Here is [issue #123](https://github.com/FelixKrueger/Bismark/issues/123)
Added option `--output_dir` to `deduplicate_bismark` so that it can be used in the Google cloud. Here is [issue #123](https://github.com/FelixKrueger/Bismark/issues/123)

### coverage2cytosine

Output files are now handled better and more consistently. Default processing now produces the following output files (with `--gzip`):
```
CpG_report.txt(.gz) or
CX_report.txt(.gz)
```

The option `--NOMe-Seq` now produces four output files (with `--gzip`):

```
NOMe.CpG_report.txt(.gz)
NOMe.CpG.cov(.gz)
NOMe.GpC_report.txt(.gz)
NOMe.GpC.cov(.gz)
```
The option `--split_by_chromosome` should work in either default, `--gc` or `--NOMe-seq` mode.

NOMe-Seq processing if now ignoring processing that were not covered by any reads.

Improved handling of the `--output_dir`, i.e. the folder will be created if it doesn't exist already and making the path absolute.

Added new option `--discordance <int>` to allow filtering for discordance pf top and bottom strand when in `--merge_CpG` mode. CpG positions for which either the top or bottom strand was not measured at all will not be assessed for discordance and hence appear in the regular 'merged_CpG_evidence.cov' file. More details in [issue #91](https://github.com/FelixKrueger/Bismark/issues/91).

Fixed context extraction for Gs at positions 1 and 2 of a chromosome/contig. Also, last cytosine positions of not covered chromosomes are now ignored in the same way as for covered chromosomes [issue #127](https://github.com/FelixKrueger/Bismark/issues/127)

### copy_files_for_release

Is now working from any location.


## Changelog for Bismark v0.18.2

### Bismark
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