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Update README.md
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alihamraoui authored Jul 12, 2024
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Expand Up @@ -78,17 +78,7 @@ Configuration for running the workflow:
| `container` | Docker container for the workflow | `'hamraouii/wf-SLSim'` |
| `docker.runOptions` | Docker run options to use | `'-u $(id -u):$(id -g)'` |

## Execution

To run the workflow with basic example:

You can simulate a realistic disribution of per barcodes UMI counts by providing in addition to the filtered counts matrix a .csv file of BC counts. AsaruSim will add a random transcrips count to fit the real distribution.
```bash
nextflow run main.nf --matrix test_data/matrix.csv \
--bc_counts test_data/test_bc.csv \
--transcriptome test_data/transcriptome.fa \

```
## Usage
User can choose among 4 ways to simulate template reads.
- use a real count matrix
- estimated the parameter from a real count matrix to simulate synthetic count matrix
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