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stepanv1 committed Jul 7, 2015
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Expand Up @@ -21,7 +21,8 @@ $R CMD INSTALL funtooNorm_N.NN.N.tar.gz

There are two functions in the package, <i>funtoonorm</i> and <i>agreement</i>.

The output of <i>funtoonorm</i> function is a normalized methylation data set of beta values and raw, not normalized beta values. As an input, user needs to have matrices with signal A and signal B extracted from Illumina IDAT files, as well as control probes signals. The function also requires a list of cell types or tissues. Besides its main purpose of normalization, <i>funtoonorm</i> can be run in a validation regime and its graphical output is used to choose optimal parameters for normalization.
The output of <i>funtoonorm</i> function is two matrices: both a normalized methylation data set of beta values and also raw, non-normalized beta values. As an input, the user needs to provide signal A and signal B matrices of data extracted from Illumina IDAT files, as well as control probes signals. The function also requires a list of cell types or tissues. Besides its main purpose of normalization, <i>funtoonorm</i> can be run in a validation mode and its graphical output is used to choose optimal parameters for normalization.


Function <i>agreement</i> accesses the performance of normalization measuring intra-replicate differences before and after normalization. It takes output of <i>funtoonorm</i> as an input.

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