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Add some documentation for the expression module
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expression | ||
========== | ||
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The `expression` module is responsible for determining gene expression levels | ||
from STAR bam and count files. Although the strandedness of the library | ||
preparation is important when determining, the module itself is strand | ||
agnostic. Instead, we take inspiration from STAR and produce output files for | ||
unstranded, forward stranded and reverse stranded libraries, and leave it to | ||
the user to select the relevant output for their samples. | ||
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Tools | ||
----- | ||
This module relies on the STAR count files in combination with a set of housekeeping genes to normalize gene expression levels. | ||
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Input | ||
----- | ||
The input for this module is one or more pairs of FastQ files per sample, specified in a PEP configuration file, as is shown below. | ||
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.. csv-table:: Example input for the expression module | ||
:delim: , | ||
:file: ../../test/pep/expression.csv | ||
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Output | ||
------ | ||
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* Three files with the normalized gene expression levels, one for each strandedness. | ||
* A single MultiQC report which contains the same data. | ||
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Configuration | ||
------------- | ||
The following options are available for the `expression` module | ||
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Example | ||
^^^^^^^ | ||
.. literalinclude:: ../../test/data/config/expression.json | ||
:language: json | ||
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Configuration options | ||
^^^^^^^^^^^^^^^^^^^^^ | ||
.. list-table:: Configuration options | ||
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* - Option | ||
- Description | ||
- Required | ||
* - housekeeping | ||
- A list of genes to use for normalizing the expression | ||
- yes | ||
* - gtf | ||
- A GTF file, to look up the ENSG for the housekeeping genes | ||
- yes | ||
* - bed | ||
- A BED file with genomic regions (genes) to quantify | ||
- yes |
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snv-indels | ||
fusion | ||
itd | ||
expression | ||
CHANGELOG |
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