This repo, should give you an excellent start. Also, at some point, be sure to consider Yeoman with RequireJS support.
First, of course, download this repo. Then, from the Terminal (assuming Node.js installed), install RequireJS.
npm install requirejs
Next, we need an easy way to deal with dependency management. We'll use Bower, from the guys at Twitter.
npm install bower
Let's now install the dependencies for this project. I'm assuming that we're building a Backbone project, so I've listed RequireJS, jQuery, Underscore, and Backbone as dependencies.
bower install
Please note that we're using the AMD versions of both Backbone and Underscore to make the setup process as easy as possible.
When ready to build the project, run:
build/build.sh
this will create a new dist directory, copy the files over, run the r.js optimizer on assets, and clean it the file structure a bit for production. Refer to app.build.jsfor configuration options.
If you're not using a preprocessor, feel free to modularize your stylesheets, and @import them into a master stylesheet. During the build process, r.js will merge these files together, so that you don't have to deal with any performance hits from using @import.