Merge pull request #4971 from Opentrons/galatea

fix(protocol/010d6c): update readme image, change aspiration height
Opentrons · Nov 27, 2023 · 37f2ecc · 37f2ecc
2 parents 329077a + 4fdea9f
commit 37f2ecc
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Showing 6 changed files with 16 additions and 11 deletions.
diff --git a/protoBuilds/010d6c/010d6c.ot2.apiv2.py.json b/protoBuilds/010d6c/010d6c.ot2.apiv2.py.json
diff --git a/protoBuilds/010d6c/README.json b/protoBuilds/010d6c/README.json
@@ -12,7 +12,7 @@
     "markdown": {
         "author": "[Opentrons](https://opentrons.com/)\n\n\n",
         "categories": "* Sample Prep\n\t* Plate Filling\n\n\n",
-        "deck-setup": "![deck](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/010d6c/ddddd.png)\n\n\n",
+        "deck-setup": "![deck](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/010d6c/Screen+Shot+2023-11-27+at+1.39.18+PM.png)\n\n\n",
         "description": "This protocol performs a Ribrogreen assay - for detailed protocol steps, please see below. There is the option to perform duplicate/triplicate plating. The csv for sample input should include `source slot, source well, destination well` in the header.\n\n\n",
         "internal": "010d6c\n",
         "labware": "* Corning 12 Reservoir 2000 \u00b5L\n* Nunc 96 Well Plate 400 \u00b5L\n* Opentrons 15 Tube Rack with eppendorf 5 mL\n* Pyramid 96 Well Plate 2000 \u00b5L\n* [Opentrons 96 Tip Rack 300 \u00b5L](https://shop.opentrons.com/collections/opentrons-tips/products/opentrons-300ul-tips)\n* [Opentrons 24 Tube Rack with NEST 2 mL Snapcap](https://shop.opentrons.com/collections/opentrons-tips/products/tube-rack-set-1)\n\n\n",

diff --git a/...lds/onsite_galatea_normalization/onsite_galatea_normalization.ot2.apiv2.ot2.apiv2.py.json b/...lds/onsite_galatea_normalization/onsite_galatea_normalization.ot2.apiv2.ot2.apiv2.py.json
diff --git a/protocols/010d6c/010d6c.ot2.apiv2.py b/protocols/010d6c/010d6c.ot2.apiv2.py
@@ -1,3 +1,9 @@
+def get_values(*names):
+    import json
+    _all_values = json.loads("""{"csv_samp":"source slot,source well,dest well\\n8,A1,A4\\n8,A1,A5","plate_standard":true,"diluent_buff_col":1,"duplicate_plating":true,"m300_mount":"left","p300_mount":"right"}""")
+    return [_all_values[n] for n in names]
+
+
 # flake8: noqa
 
 metadata = {
@@ -139,7 +145,7 @@ def Transfer_With_TT(Pipette, Source, Destination, Vol, Dispense_Top):
         calibration_vols = [1000, 750, 500, 250, 100, 50, 20, 0]
 
         p300.pick_up_tip() # Moved to outside of loop
-        p300.mix(1, 300, calibration_solution) # Moved to outside of loop
+        p300.mix(1, 300, calibration_solution.bottom(z=4)) # Moved to outside of loop
 
         for vol, well in zip(calibration_vols, diluent_buff_col):
             if vol == 0:
@@ -249,7 +255,7 @@ def Transfer_With_TT(Pipette, Source, Destination, Vol, Dispense_Top):
     for well in dispense_wells:
         m300.dispense(50, well.top())
         ctx.delay(seconds = 1)
-        m300.move_to(well.top(z = -3))
+        m300.move_to(well.top(z = 0))
         m300.touch_tip()
         ctx.delay(seconds = 1)
 
@@ -263,7 +269,7 @@ def Transfer_With_TT(Pipette, Source, Destination, Vol, Dispense_Top):
     for well in dispense_wells:
         m300.dispense(50, well.top())
         ctx.delay(seconds = 1)
-        m300.move_to(well.top(z = -3))
+        m300.move_to(well.top(z = 0))
         m300.touch_tip()
         ctx.delay(seconds = 1)
 
@@ -290,7 +296,7 @@ def Transfer_With_TT(Pipette, Source, Destination, Vol, Dispense_Top):
         for well in wells:
             m300.dispense(100, well.top())
             ctx.delay(seconds = 1)
-            m300.move_to(well.top(z = -3))
+            m300.move_to(well.top(z = 0))
             m300.touch_tip()
             ctx.delay(seconds = 1)
 

diff --git a/protocols/010d6c/README.md b/protocols/010d6c/README.md
@@ -29,7 +29,7 @@ This protocol performs a Ribrogreen assay - for detailed protocol steps, please
 
 
 ### Deck Setup
-![deck](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/010d6c/ddddd.png)
+![deck](https://opentrons-protocol-library-website.s3.amazonaws.com/custom-README-images/010d6c/Screen+Shot+2023-11-27+at+1.39.18+PM.png)
 
 
 ### Reagent Setup

diff --git a/protocols/onsite_galatea_normalization/onsite_galatea_normalization.ot2.apiv2.ot2.apiv2.py b/protocols/onsite_galatea_normalization/onsite_galatea_normalization.ot2.apiv2.ot2.apiv2.py
@@ -135,9 +135,8 @@ def pick_up_less():
 
         if transfer_vol <= 0:
             continue
-
-        p20.aspirate(transfer_vol, water)
-        p20.dispense(transfer_vol, dest_well.bottom(z=1.5))
+        p20.transfer(transfer_vol, water, dest_well.bottom(z=1.5),
+                     new_tip='never')
         p20.blow_out()
 
     p20.drop_tip()