add lid parameter

abr-testing/abr_testing/protocols/active_protocols/3_Tartrazine Protocol.py

@@ -19,62 +19,94 @@
 
 
 def add_parameters(parameters: ParameterContext) -> None:
-    """Parameters."""
-    helpers.create_single_pipette_mount_parameter(parameters)
+    """Add parameters."""
+    parameters.add_int(
+        variable_name="number_of_plates",
+        display_name="Number of Plates",
+        default=4,
+        minimum=1,
+        maximum=4,
+    )
 
 
 def run(ctx: ProtocolContext) -> None:
     """Protocol."""
-    mount_pos_50ul = ctx.params.pipette_mount  # type: ignore[attr-defined]
+    number_of_plates = ctx.params.number_of_plates  # type: ignore [attr-defined]
     # Plate Reader
     plate_reader: AbsorbanceReaderContext = ctx.load_module(
         helpers.abs_mod_str, "A3"
     )  # type: ignore[assignment]
     hs: HeaterShakerContext = ctx.load_module(helpers.hs_str, "A1")  # type: ignore[assignment]
-    hs_adapter = hs.load_adapter("opentrons_96_pcr_adapter")
+    hs_adapter = hs.load_adapter("opentrons_universal_flat_adapter")
     tube_rack = ctx.load_labware(
         "opentrons_10_tuberack_nest_4x50ml_6x15ml_conical", "C2", "Reagent Tube"
     )
     tartrazine_tube = tube_rack["A3"]
-
+    water_tube_1 = tube_rack["A4"]
+    water_tube_2 = tube_rack["B3"]
     sample_plate_1 = ctx.load_labware(
-        "nest_96_wellplate_200ul_flat", "D1", "Sample Plate 1"
+        "corning_96_wellplate_360ul_flat", "D1", "Sample Plate 1"
     )
     sample_plate_2 = ctx.load_labware(
-        "nest_96_wellplate_200ul_flat", "C1", "Sample Plate 2"
+        "corning_96_wellplate_360ul_flat", "D2", "Sample Plate 2"
     )
     sample_plate_3 = ctx.load_labware(
-        "nest_96_wellplate_200ul_flat", "B1", "Sample Plate 3"
+        "corning_96_wellplate_360ul_flat", "C1", "Sample Plate 3"
+    )
+    sample_plate_4 = ctx.load_labware(
+        "corning_96_wellplate_360ul_flat", "B1", "Sample Plate 4"
     )
-    sample_plate_list = [sample_plate_1, sample_plate_2, sample_plate_3]
+
+    sample_plate_list = [sample_plate_1, sample_plate_2, sample_plate_3, sample_plate_4]
     tiprack_50_1 = ctx.load_labware("opentrons_flex_96_tiprack_50ul", "D3")
     tiprack_50_2 = ctx.load_labware("opentrons_flex_96_tiprack_50ul", "C3")
     tiprack_50_3 = ctx.load_labware("opentrons_flex_96_tiprack_50ul", "B3")
+    tiprack_1000_1 = ctx.load_labware("opentrons_flex_96_tiprack_1000ul", "A2")
     tip_racks = [tiprack_50_1, tiprack_50_2, tiprack_50_3]
 
     # Pipette
-    p50 = ctx.load_instrument("flex_1channel_50", mount_pos_50ul, tip_racks=tip_racks)
+    p50 = ctx.load_instrument("flex_1channel_50", "left", tip_racks=tip_racks)
+    p1000 = ctx.load_instrument(
+        "flex_1channel_1000", "right", tip_racks=[tiprack_1000_1]
+    )
 
     # Probe wells
     liquid_vols_and_wells: Dict[str, List[Dict[str, Well | List[Well] | float]]] = {
-        "Tartrazine": [{"well": tartrazine_tube, "volume": 45.0}]
+        "Tartrazine": [{"well": tartrazine_tube, "volume": 45.0}],
+        "Water": [{"well": [water_tube_1, water_tube_2], "volume": 45.0}],
     }
     helpers.find_liquid_height_of_loaded_liquids(ctx, liquid_vols_and_wells, p50)
 
     i = 0
     all_percent_error_dict = {}
     cv_dict = {}
-    for sample_plate in sample_plate_list:
-        deck_locations = ["D1", "C1", "B1"]
+    vol = 0.0
+    tip_count = 0
+    for sample_plate in sample_plate_list[:number_of_plates]:
+        deck_locations = ["D1", "D2", "C1", "B1"]
+        p1000.pick_up_tip()
         for well in sample_plate.wells():
+            if vol < 45000:
+                tube_of_choice = water_tube_1
+            else:
+                tube_of_choice = water_tube_2
             p50.pick_up_tip()
+            p1000.aspirate(190, tube_of_choice)
+            p1000.air_gap(5)
+            p1000.dispense(5, well.top())
+            p1000.dispense(190, well)
+            vol += 190
             height = helpers.find_liquid_height(p50, tartrazine_tube)
             p50.aspirate(10, tartrazine_tube.bottom(z=height))
             p50.air_gap(5)
             p50.dispense(5, well.top())
             p50.dispense(10, well.bottom(z=0.5))
             p50.blow_out()
             p50.return_tip()
+            tip_count += 1
+            if tip_count >= (96 * 3):
+                p50.reset_tipracks()
+        p1000.return_tip()
         helpers.move_labware_to_hs(ctx, sample_plate, hs, hs_adapter)
         helpers.set_hs_speed(ctx, hs, 1500, 2.0, True)
         hs.open_labware_latch()
@@ -117,7 +149,6 @@ def run(ctx: ProtocolContext) -> None:
         plate_reader.open_lid()
         ctx.move_labware(sample_plate, deck_locations[i], use_gripper=True)
         i += 1
-
     # Print percent error dictionary
     ctx.comment("Percent Error: " + str(all_percent_error_dict))
     # Print cv dictionary