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ASAC

Shared code base for Amy and Adam

File Structure for RAM data

. |---VISRAM | └-------Mouse 1,2,3, etc. | └-------Day 1,2,3, etc. | └-------Behavior | └-------Calcium | | | └-------Raw | | | └-------Decompressed | | | └-------Movies | | | └------- CellMax (for rec_ files, class.txt, output | └-------Analysis (for cell traces and behavior tracking info).

File naming convention

Processing Movies

movie processing steps will be saved as hdf5 files, with the .h5 extension. They will be named according to the mouse, recording area, day, and the processing steps, as follows: m1_visp_d1_ds_nhp.h5 (after loading, mouse 1, primary visual cortex, day 1, downsampled, no hot pixels) m1_visp_d1_reg.h5 (after motion registration, mouse 1, primary visual cortex, day 1, registered) m1_visp_d1_proc.h5 (after processing, this goes into cellmax, mouse 1, primary visual cortex, day 1, processed))

Basic processing steps

Load Files, Spatially Downsample, Median Filter

Motion Registration

Temporal Downsample

Processing

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