Primer3Plus is a webinterface for Primer3

It requires the primer3, PERL and Apache.

Perl requires the module archive-zip from Adam Kennedy by calling (on Debian): aptitude install libarchive-zip-perl

It should run unaltered on windows and linux systems if you checkout from GitHub: git clone https://github.com/primer3-org/primer3plus.git primer3plus

To install copy the folder in the cgi-folder. Obtain a copy of Primer3 and install it into this folder. Adjust the settings in settings.pl to match your configuration.

To set the proper rights on linux call on commandline: ./primer3setLinuxRights.sh

Genome browser support needs a folder to store downloadable file:

• create folder genBro in the pages section.
• update the settingsfile to this path and also to have the correct html path
• do: chmod g+w geneBro chgrp www-data geneBro

Problems you may encounter: If you run encounter problems, please check the newlines to get them right for your platform (unix2dos).

Please check carefully the executable and writing rights:

• All cgi-scripts should be executable by the webserver user. chmod a+x *.cgi
• This user should have write access to all subfolders.
• All subfolders should be writable/readable by the cgi-skripts and the primer3_core executable.

Install Version 3.0.0

Primer3Plus

Primer3Plus is a webinterface for Primer3

Dependencies

Primer3Plus requires Primer3, please intall first:

https://github.com/primer3-org/primer3

Install a local copy for testing

git clone https://github.com/primer3-org/primer3plus.git

cd primer3plus

Setup and run the server

The server runs in a terminal

Install the dependencies:

sudo apt install python python-pip

pip install flask flask_cors

Start the server:

cd PATH_TO_PRIMER3PLUS/primer3plus

export PATH=$PATH:/PATH_TO_PRIMER3/src

echo $PATH

python server/server.py

Setup and run the client

The client requires a different terminal

Install the dependencies:

cd PATH_TO_PRIMER3PLUS/primer3plus/client

sudo apt install npm

npm install

Start the client:

cd PATH_TO_PRIMER3PLUS/primer3plus/client

npm run dev