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nomadscientist committed Jul 15, 2024
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34 changes: 17 additions & 17 deletions subdomains/singlecell/sections/1_beginner.yml
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- title_md: Importing files from the Single Cell Expression Atlas
description_md: >
You can import data directly from the Single Cell Expression Atlas with one tool. You will need an **experiment accession** ID, which you can find by browsing experiments at the [EBI Atlas site](https://www.ebi.ac.uk/gxa/sc/experiments). Input that **experiment accession** ID in the *Tool Parameters* box when of the following tool. Then **Run tool** to get your matrix!
<code>EBI SCXA Data Retrieval<code>
<code>EBI SCXA Data Retrieval</code>
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/retrieve_scxa/retrieve_scxa"
button_icon: tool
button_tip: Run tool
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- title_md: Importing 10X Files
description_md: >
You can find many tools for importing 10X formatted data into target datatypes.
<code>Scanpy Read10x<code>
<code>Scanpy Read10x</code>
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/scanpy_read_10x/scanpy_read_10x"
button_icon: tool
button_tip: Run tool
<code>Seurat Read10x<code>
<code>Seurat Read10x</code>
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/seurat_read10x/seurat_read10x"
button_icon: tool
button_tip: Run tool
<code>DropletUtils Read10x<code>
<code>DropletUtils Read10x</code>
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/dropletutils_read_10x/dropletutils_read_10x"
button_icon: tool
button_tip: Run tool
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- title_md: Data conversion tools
description_md: >
There are a few key tools in Galaxy for interconverting and manipulating objects in Galaxy. This list is not exhaustive.
<code>SCEasy Converter<code>
<code>SCEasy Converter</code>
This tool allows you to convert between *AnnData*, *Loom*, *Seurat*, and *Singlecellexperiment* formats.
button_link: "toolshed.g2.bx.psu.edu/repos/iuc/sceasy_convert/sceasy_convert"
button_icon: tool
button_tip: Run tool
description_md: >
<code>Manipulate AnnData<code>
<code>Manipulate AnnData</code>
Under **Function to manipulate the object** you'll find key manipulations such as *Concatenate along the observations axis* for combining AnnData objects together, *Transpose the data matrix* for help with converting formats, *Filter observations or variables* for refining or subsetting your dataset, *Adding annotations* and *Rename categories* for manipulating the metadata.
button_link: "toolshed.g2.bx.psu.edu/repos/iuc/anndata_manipulate/anndata_manipulate"
button_icon: tool
button_tip: Run tool
description_md: >
<code>AnnData Operations<code>
<code>AnnData Operations</code>
This tool allows you to flag genes (such as mitochondrial genes) as well as change names in the metadata.
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/anndata_ops/anndata_ops"
button_icon: tool
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heading_md: >
There are a few key tools for visualising single-cell data in Galaxy.
content:
- title_md: <code>Plot with Scanpy<code>
- title_md: <code>Plot with Scanpy</code>
description_md: >
<code>Plot with Scanpy<code>
<code>Plot with Scanpy</code>
This tool allows you to generate many plots, from scatterplots, to violin plots, to gene heatplots.
inputs:
- anndata
- hdf5
button_link: "toolshed.g2.bx.psu.edu/repos/iuc/scanpy_plot/scanpy_plot"
button_icon: tool
button_tip: Run tool
- title_md: <code>Scanpy PlotEmbed<code>
- title_md: <code>Scanpy PlotEmbed</code>
description_md: >
<code>Scanpy PlotEmbed<code>
<code>Scanpy PlotEmbed</code>
This tool allows you to plot embeddings like UMAPs.
inputs:
- anndata
- hdf5
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/scanpy_plot_embed/scanpy_plot_embed"
button_icon: tool
button_tip: Run tool
- title_md: <code>Scanpy PlotTrajectory<code>
- title_md: <code>Scanpy PlotTrajectory</code>
description_md: >
<code>Scanpy PlotTrajectory<code>
<code>Scanpy PlotTrajectory</code>
This tool allows you to plot trajectory data, such as PAGA, pre-calculated in an AnnData object.
inputs:
- anndata
- hdf5
button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/scanpy_plot_trajectory/scanpy_plot_trajectory"
button_icon: tool
button_tip: Run tool
- title_md: <code>Scanpy Plot dimension reduction<code>
- title_md: <code>Scanpy Plot dimension reduction</code>
description_md: >
<code>Scanpy Plot dimension reduction<code>
<code>Scanpy Plot dimension reduction</code>
This tool allows you to plot embeddings such as PCA, UMAP, and tSNE.
inputs:
- RDS with a Seurat Object
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button_link: "toolshed.g2.bx.psu.edu/repos/ebi-gxa/seurat_dim_plot/seurat_dim_plot"
button_icon: tool
button_tip: Run tool
- title_md: <code>Plot with Seurat<code>
- title_md: <code>Plot with Seurat</code>
description_md: >
<code>Plot with Seurat<code>
<code>Plot with Seurat</code>
This tool allows you to plot gene expressions, such as with Violin Plots.
inputs:
- RDS with a Seurat Object
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