Skip to content

Commit

Permalink
add tip for collection upload
Browse files Browse the repository at this point in the history
  • Loading branch information
hexylena committed Mar 8, 2024
1 parent b905865 commit b1368e1
Showing 1 changed file with 24 additions and 11 deletions.
35 changes: 24 additions & 11 deletions topics/transcriptomics/tutorials/minerva-pathways/tutorial.md
Original file line number Diff line number Diff line change
Expand Up @@ -242,6 +242,19 @@ MultiQC report. Looking at the report we see generally reasonable quality data.
> - `Add Definition``List Identifier(s)` → Select Column `B`
> - `Add Definition``URL` → Column `A`
>
> > <tip-title>Trouble entering?</tip-title>
> > 1. Press the {% icon tool %} button by **Rules**
> > 1. Paste the following JSON into the dialog:
> > ```json
> > {"rules":[],"mapping":[{"type":"collection_name","columns":[2]},{"type":"list_identifiers","columns":[1],"editing":false},{"type":"url","columns":[0]}],"genome":"hg19"}
> > ```
> > 1. Click Apply
> {: .tip}
>
> 1. At the bottom of the dialog set `Genome` to `hg19` (it is probably something like "Human Feb 2009 (GRCh37/hg19) (hg19)" but we are focused on that last parenthetical portion).
>
> 1. Click **Upload**
>
{: .hands_on}

</div>
Expand All @@ -250,17 +263,6 @@ Now we're ready to analyse the counts files. Here we'll take the feature counts

With this result in hand we're ready to do two further steps: preparing the dataset for goseq, and for analysis in MINERVA. Goseq is a tool for gene ontology enrichment analysis, and MINERVA is a tool for visualising pathway analysis.

The MINERVA dataset must be correctly formatted as a tabular dataset (`\t` separated values) like the following:

```
SYMBOL logFC P.Value adj.P.Val
TRIM25 2.07376444684004 1.2610025125617e-18 3.57368112059986e-15
ACSL1 2.90647033200259 2.71976234791064e-16 3.85390324698937e-13
NBEAL2 2.45952426389725 2.71787290816654e-14 2.56748394058132e-11
MIR150 -2.55304226607428 9.55912390273625e-14 6.74866152827879e-11
SLC2A3 2.95861349227708 1.19066011437523e-13 6.74866152827879e-11
```

<figure>
{% assign wf_counts = site | get_workflow:page, "Galaxy-Workflow-mRNA-Seq_BY-COVID_Pipeline__Analysis.ga" %}
<pre class="mermaid">
Expand All @@ -281,6 +283,17 @@ You should have a few outputs, namely the `goseq` outputs, and a table ready for

## MINERVA

The dataset prepared for MINERVA must be correctly formatted as a tabular dataset (`\t` separated values) like the following, with the dbkey set to `hg19` or `hg38`. If you've run the above workflow, this should be the case.

```
SYMBOL logFC P.Value adj.P.Val
TRIM25 2.07376444684004 1.2610025125617e-18 3.57368112059986e-15
ACSL1 2.90647033200259 2.71976234791064e-16 3.85390324698937e-13
NBEAL2 2.45952426389725 2.71787290816654e-14 2.56748394058132e-11
MIR150 -2.55304226607428 9.55912390273625e-14 6.74866152827879e-11
SLC2A3 2.95861349227708 1.19066011437523e-13 6.74866152827879e-11
```

> <hands-on-title>Visualise in MINERVA</hands-on-title>
>
> 1. **Click** to expand the final "MINERVA-Ready Table"
Expand Down

0 comments on commit b1368e1

Please sign in to comment.