Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

Sign up for GitHub

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Jump to bottom

Update tutorial.md - Assembly - Hands-0n: Quality control #5455

Open
wants to merge 3 commits into
base: main
Choose a base branch
from
Open

Update tutorial.md - Assembly - Hands-0n: Quality control #5455

Show file tree
Hide file tree
Changes from all commits
Commits
Show all changes
3 commits
Select commit Hold shift + click to select a range
f720cbe
Replacing image10
Swathi266 Oct 15, 2024
66252c1
Merge branch 'galaxyproject:main' into main
Swathi266 Oct 15, 2024
e9cf240
Update tutorial.md - Assembly - Hands-0n: Quality control
Swathi266 Oct 15, 2024
File filter

Filter by extension

Filter by extension
Conversations
Failed to load comments.
Loading
Jump to
Jump to file
Failed to load files.
Loading
Diff view
Diff view
Binary file modified topics/assembly/images/image10.png
View file
Open in desktop
Loading
Sorry, something went wrong. Reload?
Sorry, we cannot display this file.
Sorry, this file is invalid so it cannot be displayed.
11 changes: 9 additions & 2 deletions topics/sequence-analysis/tutorials/quality-control/tutorial.md
View file
Open in desktop
Original file line number Diff line number Diff line change
Expand Up @@ -148,12 +148,19 @@ It means that the fragment named `@M00970` corresponds to the DNA sequence `GTGC
>
> 1. Which ASCII character corresponds to the worst Phred score for Illumina 1.8+?
> 2. What is the Phred quality score of the 3rd nucleotide of the 1st sequence?
> 3. What is the accuracy of this 3rd nucleotide?
> 3. How to calculate the accuracy of the nucleotide base with the ASCII code /?
> 4. What is the accuracy of this 3rd nucleotide?
>
> > <solution-title></solution-title>
> > 1. The worst Phred score is the smallest one, so 0. For Illumina 1.8+, it corresponds to the `!` character.
> > 2. The 3rd nucleotide of the 1st sequence has a ASCII character `G`, which correspond to a score of 38.
> > 3. The corresponding nucleotide `G` has an accuracy of almost 99.99%
> > 3. ASCII code for / is 47
Quality score = 47-33=14
Formula to find the probability of error
P = 10 -Q/10
Probability of error = 10-14/10 = 0.03981
Therefore Accuracy = 100 - 0.03981 = 99.96%
> > 4. The corresponding nucleotide `G` has an accuracy of almost 99.99%
> >
> {: .solution }
{: .question}
Expand Down
Loading