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Add methyl-seq
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anngvu committed Sep 6, 2023
1 parent 8d6703f commit 6f80131
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Showing 2 changed files with 44 additions and 47 deletions.
80 changes: 34 additions & 46 deletions modules/classes/sequencing.yaml
Original file line number Diff line number Diff line change
@@ -1,7 +1,6 @@
classes:

ScRNA-seqLevel1:
is_a: SingleCellSequencing
Sequencing:
annotations:
requiresComponent: ''
required: false
Expand All @@ -12,34 +11,48 @@ classes:
- ParticipantID
- SampleID
- DetailedSampleID
- CryopreservedCellsinSample
- SingleCellIsolationMethod
- DissociationMethod
- SequencingPlatform
- NucleicAcidSource
- LibraryConstructionMethod
- ReadIndicator
- Read1
- Read2
- TechnicalReplicateGroup
- EndBias
- ReverseTranscriptionPrimer
- SpikeIn
- SequencingPlatform

SingleCellSequencing:
is_a: Sequencing
annotations:
requiresComponent: ''
required: false
slots:
- DissociationMethod
- CryopreservedCellsinSample
- SingleCellIsolationMethod
- TotalNumberofInputCells
- InputCellsandNuclei
- ProtocolLink
- TechnicalReplicateGroup

ScRNA-seqLevel1:
is_a: SingleCellSequencing
annotations:
requiresComponent: ''
required: false
slots:
- UMIBarcodeOffset
- UMIBarcodeLength
- MedianUMIsperCellNumber
- CellBarcodeOffset
- CellBarcodeLength
- ValidBarcodesCellNumber

ScRNA-seqLevel2:
is_a: SingleCellSequencing
annotations:
requiresComponent: ''
required: false
slots:
- Component
- Filename
- FileFormat
- ParticipantID
- SampleID
- DetailedSampleID
- ScRNAseqWorkflowType
- WorkflowVersion
- ScRNAseqWorkflowParametersDescription
Expand All @@ -58,12 +71,6 @@ classes:
requiresComponent: ''
required: false
slots:
- Component
- Filename
- FileFormat
- ParticipantID
- SampleID
- DetailedSampleID
- DataCategory
- MatrixType
- LinkedMatrices
Expand All @@ -81,12 +88,6 @@ classes:
requiresComponent: ''
required: false
slots:
- Component
- Filename
- FileFormat
- ParticipantID
- SampleID
- DetailedSampleID
- DataCategory
- MatrixType
- LinkedMatrices
Expand All @@ -104,14 +105,6 @@ classes:
requiresComponent: ''
required: false
slots:
- Component
- Filename
- FileFormat
- ParticipantID
- SampleID
- DetailedSampleID
- NucleicAcidSource
- DissociationMethod
- SingleNucleusBuffer
- SingleCellIsolationMethod
- TranspositionReaction
Expand All @@ -123,7 +116,6 @@ classes:
- ScATACseqRead2
- ScATACseqRead3
- LibraryConstructionMethod
- SequencingPlatform
- ThresholdforMinimumPassingReads
- TotalNumberofPassingNuclei
- MedianFractionofReadsinPeaks
Expand All @@ -134,27 +126,23 @@ classes:
- TotalReads
- ProtocolLink

CEL-seq2:
is_a: ScRNA-seqLevel1
BulkMethylation-seqLevel1:
is_a: Sequencing
annotations:
requiresComponent: ''
required: false
slots:
- EmptyWellBarcode
- WellIndex
- GenomicCoverageType


CellBarcodeandUMI:
CEL-seq2:
is_a: ScRNA-seqLevel1
annotations:
requiresComponent: ''
required: false
slots:
- UMIBarcodeOffset
- UMIBarcodeLength
- MedianUMIsperCellNumber
- CellBarcodeOffset
- CellBarcodeLength
- ValidBarcodesCellNumber
- EmptyWellBarcode
- WellIndex

MedianFractionofReadsinPeaks:
is_a: ScATAC-seqLevel1
Expand Down
11 changes: 10 additions & 1 deletion modules/slots.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -97,8 +97,17 @@ slots:
required: false
description: Text term to describe the result of the examination of the breast tissue specimen or fluid as related to the presence and nature of disease.
annotations:

range: BreastBiopsyProcedureFindingTypeEnum

GenomicCoverageType:
title: Genomic Coverage Type
required: true
description: Genomic coverage
enum_range:
- Whole Genome
- Targeted Genome
- Beadchip Array

Laterality:
title: Sample Laterality
required: false
Expand Down

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