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Update 0008-05-01-Gene_set_enrichment.md
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ksinghal28 authored May 1, 2024
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Expand Up @@ -162,6 +162,12 @@ cnetplot(gse_epithelial, foldChange=gene_list)

Based on these results, we could conclude that cluster 9 (putative luminal cells) have lower expression of quite a few pathways related to epithelial cell proliferation compared to cluster 12 (putative basal cells).

As an additional exercise, let's try to do an overrepresentation and GSEA analysis for the DE analysis we did on CD8 T cells. We did not have you save the file yesterday, so you can download the file first, and then use that for your analysis.

```R
download.file(url = 'http://genomedata.org/cri-workshop/reference_files/cd8tcells_de_gsea.tsv',
destfile = 'outdir_single_cell_rna/cd8tcells_de_gsea.tsv')
```



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