Create cell-free parts package #220
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Include parts in library sheet add pSB1C5 add role to those that were missing
# Conflicts: # README_distribution.md # distribution.gb # distribution.nt # distribution_synthesis_inserts.fasta
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Also added flanking seqs in the last commit. |
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This is awesome! But I think there's some confusion here. The parts (at least what I've spot checked) are mostly composites (expression devices) and not CDSes.
This is going to open a bigger topic on what do we want to actually do with the cell-free kit, and others like it.
Ex: We could isolate just the basic parts used in this kit so that these can be assembled as they want, this could get messy as some things aren't Type IIS compatible.
We could synthesize these into pSB1C3 (like the Interlab devices), so they are ready to use. Which may be closer to their intended function.
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I put everything under CDS mechanically, apologies for that. |
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This indeed will require some discussion |
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Agree! This requires more discussion. I think the current collection (composite parts) enables the user to express the designed sequences using the components in the package. It could be regarded as a kit. The user would spend more time on assembly of other parts rather than worrying about re-assembling the cell-free parts. |
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I appreciate the effort.
Does someone confirmed activity of the parts? I am afraid that iGEM17_Lethbridge used PURE-Express kit, not their purified proteins.
For example, ArgRS with C-terminal his-tag (BBa_K2443001) may not have activity because conserved C-terminual Met residue of the enzyme is buried in the structure. This is why the original PURE system paper by Shimizu et al. used an N-HisTag ArgRS plasmid I have created for my old paper.
If activities are not confirmed for the parts, I recommend sequences at
https://www.addgene.org/browse/article/28197403/
or other articles for PURE system.
Also, I propose to change the package name to “Pure cell-free system components” or “Reconstituted cell-free system components”.
@yorgomoubayed took the initiative and gathered everything in the excel file. I edited the file a bit so no errors arise, and added pSB1C5 and all parts in the libraries sheet.
We will add the flanking regions later today as well.
I think it will build nicely now, so we can merge when everything is in.
Closes #110