The official SAMtools repository can be found at: http://samtools.sourceforge.net/
- added "samtools sample" command, to sample reads from a SAM/BAM file at a given frequency.
- added "samtools qa" command, to compute the mean and median coverage, as well a histogram
from 1 to N (defined by param) containing the number of bases covered a maximum of 1X, 2X...NX.
Furthermore, "other" information is also available in the output file, namely:
- Total number of reads
- Total number of duplicates found and ignored (duplicates are "found" based on the sam flag
and are ignored in the counting of coverage)
- Percentage of unmapped reads
- Percentage of zero quality mappings
- Number of proper paired reads (based on sam flag of proper pairs)
- Percentage of proper pairs.e
- Check the write filehandle after opening for write.
- allow for user defined [lowercase] tags in header elements.
- allow the maximum memory for "samtools sort" to be specified with units.
- adjust for leading hard clip on colorspace reads.
- catches and reports an invalid BAM header, instead of segfaulting later on.
- fixes a small underflow/overflow bug in integer parsing.
- checks for a lowerbound in text entry box to avoid segfault in tview.