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Repeated sample name (SM-tag) issue with BASICFILTERING_MUTECT #24

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soccin opened this issue Oct 13, 2024 · 0 comments
Open

Repeated sample name (SM-tag) issue with BASICFILTERING_MUTECT #24

soccin opened this issue Oct 13, 2024 · 0 comments
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@soccin
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soccin commented Oct 13, 2024

Description of the bug

BASICFILTERING_MUTECT seems to also have problems with BAMs that have multiple @RG lines in them. It is not properly de-duping the SM tags. In the .command.sh file you can see the issue:

#!/bin/bash -euo pipefail
filter_mutect.py \
        --totaldepth 5 --alleledepth 3 --tnRatio 5 --variantfraction 0.01 \
        --inputVcf C_CN5Y66_T003_d03___C_CN5Y66_N901_dZ_IM7.mutect.vcf \
        --inputTxt C_CN5Y66_T003_d03___C_CN5Y66_N901_dZ_IM7.mutect.txt \
        --tsampleName s_C_CN5Y66_T003_d03
s_C_CN5Y66_T003_d03 \
        --hotspotVcf hotspot-list-union-v1-v2.vcf \
        --refFasta b37.fasta

The sample name s_C_CN5Y66_T003_d03 is repeated twice.

Command used and terminal output

I am attaching a sample input.csv file with a problem bam.

Relevant files

bug002.csv

System information

odin branch: stage, commit: 3adbfa4.

@soccin soccin added the bug Something isn't working label Oct 13, 2024
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