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Release of nf-core/demo #5

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Jun 20, 2024
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bf99c85
Adding fastp
christopher-hakkaart May 5, 2024
492e27c
Fix output names
christopher-hakkaart May 5, 2024
c295933
Update schema
christopher-hakkaart May 5, 2024
ab85083
Fixing TODOs
christopher-hakkaart May 5, 2024
86b11d1
Fixing more TODOs
christopher-hakkaart May 6, 2024
0237bd9
Fix more TODOs
christopher-hakkaart May 6, 2024
a26130a
Add metro
christopher-hakkaart May 6, 2024
aba2cb4
Remove TODO
christopher-hakkaart May 6, 2024
928b883
Fix subway
christopher-hakkaart May 6, 2024
2266191
Fix subway again
christopher-hakkaart May 6, 2024
f5d3f04
Template update for nf-core/tools version 2.13.2.dev0
mashehu May 8, 2024
a049798
Template update for nf-core/tools version 2.14.0
nf-core-bot May 8, 2024
eac360a
Template update for nf-core/tools version 2.14.1
nf-core-bot May 9, 2024
0f255c3
Merge branch 'dev' into nf-core-template-merge-2.14.1
mashehu May 9, 2024
1fc55bb
Change fastp to fq
christopher-hakkaart May 31, 2024
f1eb416
Change module to Seqtk
christopher-hakkaart May 31, 2024
bcc256a
Add skip param
christopher-hakkaart May 31, 2024
40db1d7
Update schema
christopher-hakkaart May 31, 2024
a3624b3
Adding docs
christopher-hakkaart May 31, 2024
fe9e610
Update images
christopher-hakkaart May 31, 2024
cf9e948
Prepare for sync
christopher-hakkaart May 31, 2024
3aebf65
Template update for nf-core/tools version 2.14.1
christopher-hakkaart May 31, 2024
5a91a3a
Merge branch 'TEMPLATE' into update-fq
christopher-hakkaart May 31, 2024
3cddc8b
Merge pull request #3 from nf-core/nf-core-template-merge-2.14.1
christopher-hakkaart Jun 10, 2024
070b38c
Merge branch 'dev' into update-fq
christopher-hakkaart Jun 10, 2024
39a88e7
Update output docs
christopher-hakkaart Jun 11, 2024
7ce6e7f
Update nextflow.config
christopher-hakkaart Jun 12, 2024
457e607
Merge branch 'update-fq' of https://github.com/nf-core/demo into upda…
christopher-hakkaart Jun 13, 2024
1e85529
Add change log
christopher-hakkaart Jun 19, 2024
9179262
Merge pull request #4 from nf-core/update-fq
christopher-hakkaart Jun 20, 2024
2ce1fb7
Bump version
christopher-hakkaart Jun 20, 2024
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6 changes: 1 addition & 5 deletions .editorconfig
Original file line number Diff line number Diff line change
Expand Up @@ -28,10 +28,6 @@ indent_style = unset
[/assets/email*]
indent_size = unset

# ignore Readme
[README.md]
indent_style = unset

# ignore python
# ignore python and markdown
[*.{py,md}]
indent_style = unset
2 changes: 1 addition & 1 deletion .github/PULL_REQUEST_TEMPLATE.md
Original file line number Diff line number Diff line change
Expand Up @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/demo
- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/demo/tree/master/.github/CONTRIBUTING.md)
- [ ] If necessary, also make a PR on the nf-core/demo _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository.
- [ ] Make sure your code lints (`nf-core lint`).
- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`).
- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir <OUTDIR>`).
- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir <OUTDIR>`).
- [ ] Usage Documentation in `docs/usage.md` is updated.
- [ ] Output Documentation in `docs/output.md` is updated.
Expand Down
10 changes: 5 additions & 5 deletions .github/workflows/awsfulltest.yml
Original file line number Diff line number Diff line change
Expand Up @@ -8,12 +8,12 @@ on:
types: [published]
workflow_dispatch:
jobs:
run-tower:
run-platform:
name: Run AWS full tests
if: github.repository == 'nf-core/demo'
runs-on: ubuntu-latest
steps:
- name: Launch workflow via tower
- name: Launch workflow via Seqera Platform
uses: seqeralabs/action-tower-launch@v2
# TODO nf-core: You can customise AWS full pipeline tests as required
# Add full size test data (but still relatively small datasets for few samples)
Expand All @@ -33,7 +33,7 @@ jobs:

- uses: actions/upload-artifact@v4
with:
name: Tower debug log file
name: Seqera Platform debug log file
path: |
tower_action_*.log
tower_action_*.json
seqera_platform_action_*.log
seqera_platform_action_*.json
12 changes: 6 additions & 6 deletions .github/workflows/awstest.yml
Original file line number Diff line number Diff line change
Expand Up @@ -5,13 +5,13 @@ name: nf-core AWS test
on:
workflow_dispatch:
jobs:
run-tower:
run-platform:
name: Run AWS tests
if: github.repository == 'nf-core/demo'
runs-on: ubuntu-latest
steps:
# Launch workflow using Tower CLI tool action
- name: Launch workflow via tower
# Launch workflow using Seqera Platform CLI tool action
- name: Launch workflow via Seqera Platform
uses: seqeralabs/action-tower-launch@v2
with:
workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }}
Expand All @@ -27,7 +27,7 @@ jobs:

- uses: actions/upload-artifact@v4
with:
name: Tower debug log file
name: Seqera Platform debug log file
path: |
tower_action_*.log
tower_action_*.json
seqera_platform_action_*.log
seqera_platform_action_*.json
7 changes: 2 additions & 5 deletions .github/workflows/ci.yml
Original file line number Diff line number Diff line change
Expand Up @@ -28,19 +28,16 @@ jobs:
- "latest-everything"
steps:
- name: Check out pipeline code
uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4

- name: Install Nextflow
uses: nf-core/setup-nextflow@v1
uses: nf-core/setup-nextflow@v2
with:
version: "${{ matrix.NXF_VER }}"

- name: Disk space cleanup
uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1

- name: Run pipeline with test data
# TODO nf-core: You can customise CI pipeline run tests as required
# For example: adding multiple test runs with different parameters
# Remember that you can parallelise this by using strategy.matrix
run: |
nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results
22 changes: 18 additions & 4 deletions .github/workflows/download_pipeline.yml
Original file line number Diff line number Diff line change
Expand Up @@ -14,6 +14,8 @@ on:
pull_request:
types:
- opened
- edited
- synchronize
branches:
- master
pull_request_target:
Expand All @@ -28,11 +30,14 @@ jobs:
runs-on: ubuntu-latest
steps:
- name: Install Nextflow
uses: nf-core/setup-nextflow@v1
uses: nf-core/setup-nextflow@v2

- uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
- name: Disk space cleanup
uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1

- uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
with:
python-version: "3.11"
python-version: "3.12"
architecture: "x64"
- uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7
with:
Expand Down Expand Up @@ -65,8 +70,17 @@ jobs:
- name: Inspect download
run: tree ./${{ env.REPOTITLE_LOWERCASE }}

- name: Run the downloaded pipeline
- name: Run the downloaded pipeline (stub)
id: stub_run_pipeline
continue-on-error: true
env:
NXF_SINGULARITY_CACHEDIR: ./
NXF_SINGULARITY_HOME_MOUNT: true
run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
- name: Run the downloaded pipeline (stub run not supported)
id: run_pipeline
if: ${{ job.steps.stub_run_pipeline.status == failure() }}
env:
NXF_SINGULARITY_CACHEDIR: ./
NXF_SINGULARITY_HOME_MOUNT: true
run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
6 changes: 3 additions & 3 deletions .github/workflows/fix-linting.yml
Original file line number Diff line number Diff line change
Expand Up @@ -13,7 +13,7 @@ jobs:
runs-on: ubuntu-latest
steps:
# Use the @nf-core-bot token to check out so we can push later
- uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
- uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4
with:
token: ${{ secrets.nf_core_bot_auth_token }}

Expand All @@ -32,9 +32,9 @@ jobs:
GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }}

# Install and run pre-commit
- uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
- uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
with:
python-version: 3.11
python-version: "3.12"

- name: Install pre-commit
run: pip install pre-commit
Expand Down
19 changes: 9 additions & 10 deletions .github/workflows/linting.yml
Original file line number Diff line number Diff line change
Expand Up @@ -14,13 +14,12 @@ jobs:
pre-commit:
runs-on: ubuntu-latest
steps:
- uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
- uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4

- name: Set up Python 3.11
uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
- name: Set up Python 3.12
uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
with:
python-version: 3.11
cache: "pip"
python-version: "3.12"

- name: Install pre-commit
run: pip install pre-commit
Expand All @@ -32,14 +31,14 @@ jobs:
runs-on: ubuntu-latest
steps:
- name: Check out pipeline code
uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4
uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4

- name: Install Nextflow
uses: nf-core/setup-nextflow@v1
uses: nf-core/setup-nextflow@v2

- uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
- uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
with:
python-version: "3.11"
python-version: "3.12"
architecture: "x64"

- name: Install dependencies
Expand All @@ -60,7 +59,7 @@ jobs:

- name: Upload linting log file artifact
if: ${{ always() }}
uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4
uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4
with:
name: linting-logs
path: |
Expand Down
2 changes: 1 addition & 1 deletion .github/workflows/linting_comment.yml
Original file line number Diff line number Diff line change
Expand Up @@ -11,7 +11,7 @@ jobs:
runs-on: ubuntu-latest
steps:
- name: Download lint results
uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3
uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3
with:
workflow: linting.yml
workflow_conclusion: completed
Expand Down
6 changes: 3 additions & 3 deletions .github/workflows/release-announcements.yml
Original file line number Diff line number Diff line change
Expand Up @@ -12,7 +12,7 @@ jobs:
- name: get topics and convert to hashtags
id: get_topics
run: |
curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT
echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT

- uses: rzr/fediverse-action@master
with:
Expand All @@ -25,13 +25,13 @@ jobs:

Please see the changelog: ${{ github.event.release.html_url }}

${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics
${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics

send-tweet:
runs-on: ubuntu-latest

steps:
- uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5
- uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5
with:
python-version: "3.10"
- name: Install dependencies
Expand Down
1 change: 1 addition & 0 deletions .nf-core.yml
Original file line number Diff line number Diff line change
@@ -1 +1,2 @@
repository_type: pipeline
nf_core_version: "2.14.1"
3 changes: 3 additions & 0 deletions .pre-commit-config.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -3,6 +3,9 @@ repos:
rev: "v3.1.0"
hooks:
- id: prettier
additional_dependencies:
- [email protected]

- repo: https://github.com/editorconfig-checker/editorconfig-checker.python
rev: "2.7.3"
hooks:
Expand Down
16 changes: 11 additions & 5 deletions CHANGELOG.md
Original file line number Diff line number Diff line change
Expand Up @@ -3,14 +3,20 @@
The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/)
and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html).

## v1.0dev - [date]
## 1.0.0 2024-06-19

Initial release of nf-core/demo, created with the [nf-core](https://nf-co.re/) template.
### Credits

Special thanks to the following for their reviews and assistance:

- [Maxime Garcia](https://github.com/maxulysse)
- [Friederike Hanssen](https://github.com/FriederikeHanssen)

### `Added`

### `Fixed`
- `nf-core/seqtk/trim` module
- `skip_trim` parameter

### `Dependencies`
## v1.0dev - 2024-05-5

### `Deprecated`
Initial release of nf-core/demo, created with the [nf-core](https://nf-co.re/) template.
2 changes: 2 additions & 0 deletions CITATIONS.md
Original file line number Diff line number Diff line change
Expand Up @@ -14,6 +14,8 @@

> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].

- [seqtk](https://github.com/lh3/seqtk)

- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)

> Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
Expand Down
39 changes: 12 additions & 27 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -13,52 +13,41 @@
[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)
[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)
[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)
[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/demo)
[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/demo)

[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23demo-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/demo)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)

## Introduction

**nf-core/demo** is a bioinformatics pipeline that ...
**nf-core/demo** is a simple nf-core style bioinformatics pipeline for workshops and demonstrations. It was created using the nf-core template and is designed to run quickly using small test data files.

<!-- TODO nf-core:
Complete this sentence with a 2-3 sentence summary of what types of data the pipeline ingests, a brief overview of the
major pipeline sections and the types of output it produces. You're giving an overview to someone new
to nf-core here, in 15-20 seconds. For an example, see https://github.com/nf-core/rnaseq/blob/master/README.md#introduction
-->
![nf-core/demo metro map](docs/images/nf-core-demo-subway.png)

<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. -->
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->

1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
1. Read QC ([`FASTQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
2. Adapter and quality trimming ([`SEQTK_TRIM`](https://github.com/lh3/seqtk))
3. Present QC for raw reads ([`MULTIQC`](http://multiqc.info/))

## Usage

> [!NOTE]
> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.

<!-- TODO nf-core: Describe the minimum required steps to execute the pipeline, e.g. how to prepare samplesheets.
Explain what rows and columns represent. For instance (please edit as appropriate):

First, prepare a samplesheet with your input data that looks as follows:

`samplesheet.csv`:

```csv
sample,fastq_1,fastq_2
CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz
SAMPLE1_PE,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample1_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample1_R2.fastq.gz
SAMPLE2_PE,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample2_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample2_R2.fastq.gz
SAMPLE3_SE,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample1_R1.fastq.gz,
SAMPLE3_SE,https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/illumina/amplicon/sample2_R1.fastq.gz,
```

Each row represents a fastq file (single-end) or a pair of fastq files (paired end).

-->

Now, you can run the pipeline using:

<!-- TODO nf-core: update the following command to include all required parameters for a minimal example -->

```bash
nextflow run nf-core/demo \
-profile <docker/singularity/.../institute> \
Expand All @@ -80,11 +69,9 @@ For more details about the output files and reports, please refer to the

## Credits

nf-core/demo was originally written by Christopher Hakkaart.

We thank the following people for their extensive assistance in the development of this pipeline:
nf-core/demo was originally written by Chris Hakkaart ([@christopher-hakkaart](https://github.com/christopher-hakkaart)).

<!-- TODO nf-core: If applicable, make list of people who have also contributed -->
<!-- We thank the following people for their extensive assistance in the development of this pipeline: -->

## Contributions and Support

Expand All @@ -97,8 +84,6 @@ For further information or help, don't hesitate to get in touch on the [Slack `#
<!-- TODO nf-core: Add citation for pipeline after first release. Uncomment lines below and update Zenodo doi and badge at the top of this file. -->
<!-- If you use nf-core/demo for your analysis, please cite it using the following doi: [10.5281/zenodo.XXXXXX](https://doi.org/10.5281/zenodo.XXXXXX) -->

<!-- TODO nf-core: Add bibliography of tools and data used in your pipeline -->

An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.

You can cite the `nf-core` publication as follows:
Expand Down
1 change: 0 additions & 1 deletion assets/methods_description_template.yml
Original file line number Diff line number Diff line change
Expand Up @@ -3,7 +3,6 @@ description: "Suggested text and references to use when describing pipeline usag
section_name: "nf-core/demo Methods Description"
section_href: "https://github.com/nf-core/demo"
plot_type: "html"
## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline
## You inject any metadata in the Nextflow '${workflow}' object
data: |
<h4>Methods</h4>
Expand Down
4 changes: 2 additions & 2 deletions assets/multiqc_config.yml
Original file line number Diff line number Diff line change
@@ -1,7 +1,7 @@
report_comment: >
This report has been generated by the <a href="https://github.com/nf-core/demo/tree/dev" target="_blank">nf-core/demo</a>
This report has been generated by the <a href="https://github.com/nf-core/demo/releases/tag/1.0.0" target="_blank">nf-core/demo</a>
analysis pipeline. For information about how to interpret these results, please see the
<a href="https://nf-co.re/demo/dev/docs/output" target="_blank">documentation</a>.
<a href="https://nf-co.re/demo/1.0.0/docs/output" target="_blank">documentation</a>.
report_section_order:
"nf-core-demo-methods-description":
order: -1000
Expand Down
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